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Literature summary for 1.21.99.5 extracted from
- Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli (1998), J. Bacteriol., 180, 4140-4145.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of pceA gene in Escherichia coli BL21 | Sulfurospirillum multivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Sulfurospirillum multivorans | 5737 | - |
| cytoplasmic membrane | - |
Sulfurospirillum multivorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | cobalamin-containing enzyme | Sulfurospirillum multivorans |  |
| Cobalt | - |
Sulfurospirillum multivorans | |
| Fe2+ | two Fe/S clusters | Sulfurospirillum multivorans | |
| Fe2+ | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| Iron-sulfur cluster | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| iron-sulfur clusters | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| S | two Fe/S clusters | Sulfurospirillum multivorans | |
| S | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55890 | - |
amino acid sequence analysis | Sulfurospirillum multivorans |
| 57000 | - |
processed enzyme, pceA' gene product, SDS-PAGE | Sulfurospirillum multivorans |
| 58000 | - |
gel filtration | Sulfurospirillum multivorans |
| 61000 | - |
unprocessed enzyme, expressed in Escherichia coli, SDS-PAGE | Sulfurospirillum multivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | Sulfurospirillum multivorans | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurospirillum multivorans | - |
strictly anaerobic, gram-negative bacterium | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | modified by truncation of the first 30 amino acids | Sulfurospirillum multivorans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurospirillum multivorans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | chemiosmotic mechanism | Sulfurospirillum multivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | - |
Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | - |
Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | corrinoid probably noncavalently bound to enzyme | Sulfurospirillum multivorans | |
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