Application | Comment | Organism |
---|---|---|
agriculture | suitable for tetrachloroethene bioremediation | Desulfitobacterium hafniense |
General Stability | Organism |
---|---|
oxygen-sensitive with half-life of approximately 50 min | Desulfitobacterium hafniense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by ammonium ions | Desulfitobacterium hafniense | |
Propyl iodide | 1-iodopropane; 1-iodopropane, photoreversible inactivation | Desulfitobacterium hafniense | |
trichloroethene | substrate inhibition, higher concentrations of trichloroethene: inhibition of PCE dehalogenase, Ki: 1.3 mM | Desulfitobacterium hafniense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme kinetics and kinetic parameters | Desulfitobacterium hafniense | |
0.004 | - |
trichloroethene | - |
Desulfitobacterium hafniense | |
0.01 | - |
tetrachloroethene | - |
Desulfitobacterium hafniense | |
0.3 | - |
methyl viologen | at a tetrachloroethene concentration of 1 mM | Desulfitobacterium hafniense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | membrane-bound | Desulfitobacterium hafniense | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | dicyano-cob(III)alamin | Desulfitobacterium hafniense | |
Cobalt | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
Cobalt | cobalt content: 1.0 mol of cobalt/mol of protein | Desulfitobacterium hafniense | |
Fe2+ | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
Fe2+ | two Fe/S clusters | Desulfitobacterium hafniense | |
Fe2+ | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
Iron-sulfur cluster | iron-sulfur protein | Desulfitobacterium hafniense | |
Iron-sulfur cluster | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
Iron-sulfur cluster | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
iron-sulfur clusters | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
iron-sulfur clusters | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
NH4+ | no effect, no activation by ammonium ions | Desulfitobacterium hafniense | |
S | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
S | two Fe/S clusters | Desulfitobacterium hafniense | |
S | two Fe4-S4 clusters | Desulfitobacterium hafniense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE | Desulfitobacterium hafniense |
200000 | - |
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, gel filtration | Desulfitobacterium hafniense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + reduced acceptor | Desulfitobacterium hafniense | - |
trichloroethene + chloride + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfitobacterium hafniense | - |
strictly anaerobic, gram-positive bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Desulfitobacterium hafniense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Desulfitobacterium hafniense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity | Desulfitobacterium hafniense | ? | - |
? | |
additional information | enzyme quite specific | Desulfitobacterium hafniense | ? | - |
? | |
additional information | very high substrate specificity | Desulfitobacterium hafniense | ? | - |
? | |
additional information | no activity with other chloroethenes or chloromethanes or chlorinated aromatic compounds, no activity with carbon tetrachloride, 1,1-dichloroethene, trans-1,2-dichloroethene, cis-1,2-dichloroethene, 3-chlorobenzoate, 3-chloro-4-hydroxyphenol, pentachlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol | Desulfitobacterium hafniense | ? | - |
? | |
tetrachloroethene + reduced acceptor | - |
Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
trichloroethene + chloride + methyl viologen | - |
Desulfitobacterium hafniense | tetrachloroethene + reduced methyl viologen | - |
r | |
trichloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Desulfitobacterium hafniense | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
trichloroethene + reduced acceptor | 70% of activity compared with tetrachloroethene | Desulfitobacterium hafniense | cis-1,2-dichloroethene + chloride + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, probably a monomeric 65-kDa protein, SDS-PAGE | Desulfitobacterium hafniense |
More | membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo | Desulfitobacterium hafniense |
trimer | 3 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE | Desulfitobacterium hafniense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
approximately | Desulfitobacterium hafniense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
approximately | Desulfitobacterium hafniense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
corrinoid | corrinoid protein | Desulfitobacterium hafniense | |
corrinoid | dicyano-cobalamin | Desulfitobacterium hafniense | |
corrinoid | dicyano-cob(III)alamin | Desulfitobacterium hafniense | |
corrinoid | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense |