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Literature summary for 1.21.99.5 extracted from

  • Schumacher, W.; Holliger, C.; Zehnder, A.J.B.; Hagen, W.R.
    Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus (1997), FEBS Lett., 409, 421-425.
    View publication on PubMed
Search for more literature for 1.21.99.5

Inhibitors

Inhibitors Comment Organism Structure
Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation Dehalobacter restrictus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane membrane-bound Dehalobacter restrictus
-
-
cytoplasmic membrane cytoplasmic membrane Dehalobacter restrictus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cobalamin cob(II)alamin Dehalobacter restrictus
Cobalamin in base-off configuration Dehalobacter restrictus
Cobalt probably present in the 2+ oxidation state Dehalobacter restrictus
Fe2+ two Fe4-S4 clusters Dehalobacter restrictus
Iron-sulfur cluster two iron-sulfur cubane cofactors Dehalobacter restrictus
Iron-sulfur cluster two Fe4-S4 clusters Dehalobacter restrictus
iron-sulfur clusters two Fe4-S4 clusters Dehalobacter restrictus
S two Fe4-S4 clusters Dehalobacter restrictus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Dehalobacter restrictus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Dehalobacter restrictus PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond ?
-
 ?
tetrachloroethene + reduced acceptor Dehalobacter restrictus PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond trichloroethene + chloride + acceptor
-
 ?
trichloroethene + reduced acceptor Dehalobacter restrictus
-
 cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Dehalobacter restrictus
-
 DSMZ 9455T
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dehalobacter restrictus

Reaction

Reaction Comment Organism Reaction ID
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor mechanism Dehalobacter restrictus
a
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor chemiosmotic mechanism Dehalobacter restrictus
a
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor thermodynamics Dehalobacter restrictus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
-
 Dehalobacter restrictus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information physiological electron donor is not known Dehalobacter restrictus ?
-
 ?
additional information PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond Dehalobacter restrictus ?
-
 ?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Dehalobacter restrictus trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond Dehalobacter restrictus trichloroethene + chloride + acceptor
-
 ?
trichloroethene + reduced acceptor
-
 Dehalobacter restrictus cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Dehalobacter restrictus

Cofactor

Cofactor Comment Organism Structure
vitamin B12
-
 Dehalobacter restrictus