Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.99.4 extracted from

  • Koehrle, J.
    Iodothyronine deiodinases (2002), Methods Enzymol., 347, 125-167.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3,3',5-triiodo-L-thyronine induces isozyme type I vertebrata
atrial natriuretic protein induces isozyme type II via cGMP vertebrata
beta-adrenergic agonists stimulates isozyme type II vertebrata
C-type natriuretic protein induces isozyme type II via cGMP vertebrata
cAMP
-
Rattus norvegicus
cAMP induces isozyme type I in thyroid gland only, induces isozyme type II vertebrata
Epidermal growth factor
-
Rattus norvegicus
fibroblast growth factor induces isozymes type II, and I vertebrata
additional information expression and function of the deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents Homo sapiens
Nicotine stimulates isozyme type II vertebrata
Phorbol esters induce isozyme type II via protein kinase C vertebrata
retinoic acid
-
Rattus norvegicus
retinoic acid stimulates isozyme type I vertebrata
Se2+
-
Rattus norvegicus
Se2+ severe selenium deficiency leads to a decrease in activity of isozyme type I in liver, kidney, and several other organs, but not in the thyroid gland, the central nervous system, and several other endrocrine organs Homo sapiens
Se2+ stimulates isozyme type I, absolutely required, isozymes of the deiodinase enzyme family are selenoproteins vertebrata
testosterone stimulates isozyme type I in liver vertebrata
thyroid stimulating hormone induces isozyme type I vertebrata

Application

Application Comment Organism
medicine expression and function of the 5-deiodinase and 5’-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
isozyme type I, mapping on chromosome 1p32-p33, DNA sequence analysis Homo sapiens
isozyme type I, mapping on chromosome 4 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
3,3',5'-triiodo-L-thyronine
-
Homo sapiens
3,3',5'-triiodo-L-thyronine isozyme type II, rapid inactivation in brain Rattus norvegicus
3,3',5'-triiodo-L-thyronine inhibits expression of isozyme type II vertebrata
aurothioglucose
-
Homo sapiens
aurothioglucose isoyzme type I vertebrata
Ca2+ inhibits expression of isozyme type I in thyroid gland vertebrata
dexamethasone inhibits expression of isozyme type I vertebrata
iodoacetate isozyme type I vertebrata
iopanoic acid both isozyme type I and type II Homo sapiens
iopanoic acid both isozyme type I and type II vertebrata
L-thyroxine isozyme type II vertebrata
additional information expression and function of the 5-deiodinase and 5’-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology Homo sapiens
propylthiouracil isozyme type I vertebrata
thyroxine isozyme type II, rapid inactivation in brain Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information review on enzyme isoforms type I and II and 5-deiodinase, type III Homo sapiens
additional information
-
additional information review on enzyme isoforms type I and II and 5-deiodinase, type III Rattus norvegicus
additional information
-
additional information review on enzyme isoforms type I and II and 5-deiodinase, type III Bos taurus
additional information
-
additional information review on enzyme isoforms type I and II and 5-deiodinase, type III vertebrata

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum liver, smooth and rough, isozyme type I, at the cytosolic surface vertebrata 5783
-
endoplasmic reticulum anterior pituitary gland, isozyme type I Bos taurus 5783
-
microsome
-
Mus musculus
-
-
microsome
-
Homo sapiens
-
-
microsome
-
vertebrata
-
-
microsome isozyme type I, liver Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus 5739
-
mitochondrion
-
vertebrata 5739
-
additional information membrane association is not essential for maintenance of functional activity Rattus norvegicus
-
-
additional information subcellular localization of the different isozyme types in different tissues, overview vertebrata
-
-
plasma membrane at the inner leaflet of the basolateral plasma membrane, isozyme type I, kidney vertebrata 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2 * 27000, isozyme type I Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3',5'-triiodo-L-thyronine + AH2 Mus musculus isozyme type I 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 Homo sapiens isozyme type I 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 Rattus norvegicus isozyme type I 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 vertebrata isozyme type I 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
L-thyroxine + AH2 Rattus norvegicus isozymes type II and I, production of 3,3',5-triiodo-L-thyronine 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 vertebrata isozymes type II and I, production of 3,3',5-triiodo-L-thyronine 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 Mus musculus
-
3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2 Homo sapiens
-
3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2 Rattus norvegicus
-
3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2 vertebrata
-
3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2 Mus musculus isozymes type II and I, production of 3,3',5-triiodo-L-thyronine 3,5,3'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 Homo sapiens isozymes type II and I, production of 3,3',5-triiodo-L-thyronine 3,5,3'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
type I and type II enzyme isoforms
-
Homo sapiens
-
type I and type II enzyme isoforms
-
Mus musculus
-
type I and type II enzyme isoforms
-
Rattus norvegicus
-
type I and type II enzyme isoforms
-
vertebrata
-
type I and type II enzyme isoforms
-

Reaction

Reaction Comment Organism Reaction ID
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor isozyme type I, reaction mechanism, both 5’-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site Mus musculus
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor isozyme type I, reaction mechanism, both 5’-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site Rattus norvegicus
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor isozyme type I, reaction mechanism, both 5’-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site Bos taurus
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor isozyme type I, reaction mechanism, both 5’-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site vertebrata

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
Rattus norvegicus
-
adipocyte isozyme type II Mus musculus
-
adipocyte isozyme type II vertebrata
-
brain isozyme type II Mus musculus
-
brain isozyme type II vertebrata
-
brown adipose tissue isozyme type II Mus musculus
-
brown adipose tissue isozyme type II Rattus norvegicus
-
brown adipose tissue isozyme type II vertebrata
-
central nervous system isozyme type I Homo sapiens
-
epithelium of kidney, isozyme type I Mus musculus
-
epithelium of kidney, isozyme type I vertebrata
-
glial cell isozyme type II Mus musculus
-
glial cell isozyme type II vertebrata
-
harderian gland isozyme type II vertebrata Mus musculus
-
harderian gland isozyme type II vertebrata vertebrata
-
heart isozyme type I Mus musculus
-
heart isozyme type I vertebrata
-
hepatocyte
-
Homo sapiens
-
kidney isozyme type I Mus musculus
-
kidney isozyme type I Homo sapiens
-
kidney isozyme type I vertebrata
-
liver isozyme type I Mus musculus
-
liver isozyme type I Homo sapiens
-
liver isozyme type I Rattus norvegicus
-
liver isozyme type I vertebrata
-
additional information severe selenium deficiency leads to a decrease in activity of isozyme type I in liver, kidney, and several other organs, but not in the thyroid gland, the central nervous system, and several other endrocrine organs Homo sapiens
-
pineal gland isozyme type II Mus musculus
-
pineal gland isozyme type II vertebrata
-
pituitary gland isozyme type I Rattus norvegicus
-
pituitary gland anterior, isozyme type I Bos taurus
-
pituitary gland anterior, isozyme type II, isozyme type I Mus musculus
-
pituitary gland anterior, isozyme type II, isozyme type I vertebrata
-
placenta isozyme type II Mus musculus
-
placenta isozyme type II Rattus norvegicus
-
skin
-
Rattus norvegicus
-
skin isozyme type II Mus musculus
-
skin isozyme type II vertebrata
-
tanycyte isozyme type II Mus musculus
-
tanycyte isozyme type II vertebrata
-
thymus isozyme type II Mus musculus
-
thymus isozyme type II vertebrata
-
thyroid gland
-
Homo sapiens
-
thyroid gland isozyme type I Rattus norvegicus
-
thyroid gland isozyme types I and II Mus musculus
-
thyroid gland isozyme types I and II vertebrata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
overview on assay methods Mus musculus
additional information
-
overview on assay methods Rattus norvegicus
additional information
-
overview on assay methods Bos taurus
additional information
-
overview on assay methods vertebrata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I Mus musculus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I Homo sapiens 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I Rattus norvegicus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I vertebrata 3,3'-diiodo-L-thyronine + iodide + A + H+
-
?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I: degradation of 3,3',5'-triiodo-L-thyronine Mus musculus 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I: degradation of 3,3',5'-triiodo-L-thyronine Rattus norvegicus 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I, degradation of 3,3',5'-triiodo-L-thyronine Homo sapiens 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I, degradation of 3,3',5'-triiodo-L-thyronine Bos taurus 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5'-triiodo-L-thyronine + AH2 isozyme type I, degradation of 3,3',5'-triiodo-L-thyronine vertebrata 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 isozymes type II and I, production of 3,3',5-triiodo-L-thyronine Rattus norvegicus 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 isozymes type II and I, production of 3,3',5-triiodo-L-thyronine vertebrata 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2
-
Mus musculus 3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2
-
Homo sapiens 3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2
-
Rattus norvegicus 3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2
-
Bos taurus 3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2
-
vertebrata 3,3',5-triiodo-L-thyronine + iodide + A + H+ from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active ?
L-thyroxine + AH2 isozyme type II Mus musculus 3,3',5-triiodo-L-thyronine + iodide + A + H+ hormonally active ?
L-thyroxine + AH2 isozyme type II Homo sapiens 3,3',5-triiodo-L-thyronine + iodide + A + H+ hormonally active ?
L-thyroxine + AH2 isozyme type II Bos taurus 3,3',5-triiodo-L-thyronine + iodide + A + H+ hormonally active ?
L-thyroxine + AH2 isozyme type II vertebrata 3,3',5-triiodo-L-thyronine + iodide + A + H+ hormonally active ?
L-thyroxine + AH2 isozyme type II Rattus norvegicus 3,5,3'-triiodo-L-thyronine + iodide + A + H+ hormonally active ?
L-thyroxine + AH2 isozymes type II and I, production of 3,3',5-triiodo-L-thyronine Mus musculus 3,5,3'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 isozymes type II and I, production of 3,3',5-triiodo-L-thyronine Homo sapiens 3,5,3'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
additional information selenium-level of cells influences the enzyme activity Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27000, isozyme type I Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus
37
-
assay at Bos taurus
37
-
assay at vertebrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
dithioerythritol
-
Homo sapiens
dithioerythritol can be substituted by dithiothreitol, isozyme type II, 5-20 mM, isozyme type I, 2-5 mM Rattus norvegicus
dithioerythritol can be substituted by dithiothreitol, isozyme type II, 5-20 mM, isozyme type I, 2-5 mM vertebrata
dithiothreitol can be substituted by dithioerythritol, isozyme type II, 5-20 mM, isozyme type I, 2-5 mM Rattus norvegicus
dithiothreitol can be substituted by dithioerythritol, isozyme type II, 5-20 mM, isozyme type I, 2-5 mM vertebrata
additional information no activity with glutathione or thioredoxin in vivo, isozyme type I Rattus norvegicus
additional information no activity with glutathione or thioredoxin in vivo, isozyme type I vertebrata