KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
L-3,5',3'-triiodothyronine | pH 7.4, 37°C, isozyme Dio2 | Homo sapiens | |
0.006 | - |
L-3,5',3'-triiodothyronine | pH 7.4, 37°C, isozyme Dio3 | Homo sapiens | |
0.57 | - |
L-3,5',3'-triiodothyronine | pH 7.4, 37°C, isozyme Dio1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview | ? | - |
? | |
additional information | Homo sapiens | thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
three deiodinase isozymes Dio1, Dio2, and Dio3 | - |
Mus musculus | - |
three deiodinase isozymes Dio1, Dio2, and Dio3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ECC-1 cell | isozyme Dio3 | Homo sapiens | - |
Hep-G2 cell | isozyme Dio1 | Homo sapiens | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
MSTO-211H cell | isozyme Dio2 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3',5'-diiodothyronine + AH2 | phenolic ring deiodination of 3',5'-T2 by Dio2 | Mus musculus | L-5'-iodothyronine + iodide + A + H+ | - |
? | |
L-3',5'-diiodothyronine + AH2 | phenolic ring deiodination of 3',5'-T2 by Dio2 | Homo sapiens | L-5'-iodothyronine + iodide + A + H+ | - |
? | |
L-3,5,3',5'-tetraiodothyronine + AH2 | i.e. thyroxine, reaction of isozyme Dio3 | Homo sapiens | L-3,5',3'-triiodothyronine + iodide + A + H+ | i.e. reverse triiodothyrosine | ? | |
L-3,5,3'-triiodothyronine + AH2 | 5-deionination by isozyme Dio1 | Mus musculus | L-3,3'-diiodothyronine + iodide + A + H+ | - |
? | |
L-3-iodothyronine + AH2 | tyrosyl ring deiodination of 3-iodothyronamine by isozyme Dio3 | Mus musculus | L-thyronine + iodide + A + H+ | - |
? | |
L-3-iodothyronine + AH2 | tyrosyl ring deiodination of 3-iodothyronamine by isozyme Dio3 | Homo sapiens | L-thyronine + iodide + A + H+ | - |
? | |
additional information | thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview | Mus musculus | ? | - |
? | |
additional information | thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview | Homo sapiens | ? | - |
? | |
additional information | isozyme Dio1 exhibits both phenolic and tyrosyl ring deiodination activity while isozyme Dio2 catalyzes only deiodinations of the phenolic ring, isozyme Dio3 catalyzes only deiodinations of the tyrosyl ring. T3, 3,3'-T2, and 3,5-T2 are also not deiodinated by Dio1 from HepG2 lysates | Homo sapiens | ? | - |
? | |
additional information | isozyme Dio1 from mouse liver membrane fractions catalyzes a weak 3'-deiodination of 3,3'-T2 as well as faint 5-deiodinations of both T3 and 3,5-T2 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deiodinase | - |
Mus musculus |
deiodinase | - |
Homo sapiens |
Dio | - |
Mus musculus |
Dio | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |