Cloned (Comment) | Organism |
---|---|
isozyme type III, expression of wild-type and active site mutants in COS cells, 50fold higher expression level for the mutants than for the wild-type enzyme | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
SeC144A | site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, enzymatically inactive | Homo sapiens |
SeC144C | site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, 5fold increased Km for 3,3',5-triiodo-L-thyronine and 100fold increased Km for 3,3',5,5'-tetraiodo-L-thyronine compared to the wild-type, 2-6fold reduced turnover | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000028 | - |
3,3',5-triiodo-L-thyronine | native wild-type, pH 7.2, 37°C, in presence of 1 mM DTT | Homo sapiens | |
0.0000031 | - |
3,3',5-triiodo-L-thyronine | recombinant wild-type, pH 7.2, 37°C, in presence of 0.3 mM DTT | Homo sapiens | |
0.0000035 | - |
L-thyroxine | recombinant wild-type, pH 7.2, 37°C, in presence of 3 mM DTT | Homo sapiens | |
0.0000042 | - |
3,3',5-triiodo-L-thyronine | recombinant mutant SeC144C, pH 7.2, 37°C, in presence of 0.3 mM DTT | Homo sapiens | |
0.000008 | - |
L-thyroxine | native wild-type, pH 7.2, 37°C, in presence of 10 mM DTT | Homo sapiens | |
0.000265 | - |
L-thyroxine | recombinant mutant SeC144C, pH 7.2, 37°C, in presence of 1 mM DTT | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | isozyme type III | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, isozyme type III, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | Homo sapiens | selenocysteine residue in the active site is essential for efficient inner ring deiodination | 3,3'-diiodo-L-thyronine + iodide + A + H+ | i.e. 3,3'-diiodo-L-thyronine | ? | |
3,3',5-triiodo-L-thyronine + AH2 | Homo sapiens | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | 3,3'-diiodo-L-thyronine + iodide + A + H+ | i.e. 3,3'-diiodo-L-thyronine | ? | |
L-thyroxine + AH2 | Homo sapiens | 5-deiodinase activity | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | |
L-thyroxine + AH2 | Homo sapiens | selenocysteine residue in the active site is absolutely essential for efficient inner ring deiodination | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55073 | - |
- |
Homo sapiens | P55073 | isozyme type III | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3,3',5'-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | all 3 isozyme types contain a selenocysteine residue at the active site | Homo sapiens | |
3,3',5'-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | inner ring deiodination | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | isozyme type III | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | isozyme type III | Homo sapiens | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodo-L-thyronine + AH2 | selenocysteine residue in the active site is essential for efficient inner ring deiodination | Homo sapiens | 3,3'-diiodo-L-thyronine + iodide + A + H+ | i.e. 3,3'-diiodo-L-thyronine | ? | |
3,3',5-triiodo-L-thyronine + AH2 | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Homo sapiens | 3,3'-diiodo-L-thyronine + iodide + A + H+ | i.e. 3,3'-diiodo-L-thyronine | ? | |
L-thyroxine + AH2 | isozyme type III | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | 5-deiodinase activity | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | 5-deiodinase activity | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | |
L-thyroxine + AH2 | selenocysteine residue in the active site is absolutely essential for efficient inner ring deiodination | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, isozyme type III, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
dithiothreitol | reducing cofactor | Homo sapiens |