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Literature summary for 1.21.99.3 extracted from

  • Sanders, J.P.; Van der Geyten, S.; Kaptein, E.; Darras, V.M.; Kuhn, E.R.; Leonard, J.L.; Visser, T.J.
    Cloning and characterization of type III iodothyronine deiodinase from the fish Oreochromis niloticus (1999), Endocrinology, 140, 3666-3673.
    View publication on PubMed
Search for more literature for 1.21.99.3

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells Oreochromis niloticus

Inhibitors

Inhibitors Comment Organism Structure
aurothioglucose recombinant and native isozyme type III Oreochromis niloticus
iodoacetate recombinant and native isozyme type III Oreochromis niloticus
propylthiouracil slight inhibition of recombinant and native isozyme type III Oreochromis niloticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
 3,3',5-triiodo-L-thyronine pH 7.2, 37°C Oreochromis niloticus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome isozyme type III Oreochromis niloticus
-
-

Organism

Organism UniProt Comment Textmining
Oreochromis niloticus Q9I966 isozyme type III
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from COS-1 cells Oreochromis niloticus

Source Tissue

Source Tissue Comment Organism Textmining
brain isozyme type III Oreochromis niloticus
-
gill gene expression Oreochromis niloticus
-
gut very low activity Oreochromis niloticus
-
gut isozyme type III Oreochromis niloticus
-
kidney low activity Oreochromis niloticus
-
kidney isozyme type III Oreochromis niloticus
-
liver low activity Oreochromis niloticus
-
liver isozyme type III Oreochromis niloticus
-
muscle very low activity Oreochromis niloticus
-
muscle isozyme type III Oreochromis niloticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5-triiodo-L-thyronine + AH2 best substrate Oreochromis niloticus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
 ?
3,3',5-triiodo-L-thyronine + AH2 isozyme type III Oreochromis niloticus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
 ?
3,3',5-triiodo-L-thyronine sulfate + AH2 isozyme type III Oreochromis niloticus 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
-
 ?
3,3',5-triiodo-L-thyronine sulfate + AH2 about 14% of the activity with 3,3',5-triiodo-L-thyronine Oreochromis niloticus 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
-
 ?
L-thyroxine + AH2 very low activity Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
L-thyroxine + AH2 isozyme type III Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
L-thyroxine + AH2 5-deiodinase activity Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oreochromis niloticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Oreochromis niloticus

Cofactor

Cofactor Comment Organism Structure
dithiothreitol i.e. DTT Oreochromis niloticus