Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | absolutely required for protein C activity | Acetoanaerobium sticklandii | |
thiols | such as dithiothreitol required for decomposition of acetyl phosphate by protein C | Acetoanaerobium sticklandii |
General Stability | Organism |
---|---|
the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited by alkylation selenoprotein A alkylated at pH 6 with bromoacetate is active as a component of the enzyme complex | Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for decomposition of acetyl phosphate by protein C | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
molecular mass is depending on the salt concentration present | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii | - |
glycine + phosphate + thioredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of protein C of enzyme | Acetoanaerobium sticklandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | the protein C component catalyses the arsenate-dependent decomposition of acetyl phosphate | Acetoanaerobium sticklandii | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | the protein component C serves as the acetyl group acceptor in the overall reaction | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | reduction of glycine action is coupled with formation of ATP from ADP | Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | 64 | protein C inactivated | Acetoanaerobium sticklandii |