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Literature summary for 1.21.4.1 extracted from
- In vitro processing of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: formation of a pyruvoyl group from a cysteine residue (2001), Eur. J. Biochem., 268, 3538-3544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of the genes encoding D-proline reductase, 4.8 kb EcoRI fragment containing the genes prdA and prdB isolated and sequenced, prdAcodes for a 68 kDa protein, posttranslationally cleaved to the 45 kDa and 23 kDa subunits, prdB encodes the 26 kDa subunit | Acetoanaerobium sticklandii |
| D-proline reductase proproteins cloned and overexpressed in Escherichia coli XL2 blue and BL21 | Acetoanaerobium sticklandii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |
| 45000 | - |
10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
strain ATCC12662 identical with strain DSM 519 T | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-proline + dithiothreitol | Stickland reaction | Acetoanaerobium sticklandii | 5-amino pentanoic acid + ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | 10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |
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Release 2023.1 (January 2023)
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