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Literature summary for 1.21.4.1 extracted from

  • Seto, B.; Stadtman, T.C.
    Purification and properties of proline reductase from Clostridium sticklandii (1976), J. Biol. Chem., 251, 2435-2439.
    View publication on PubMed
Search for more literature for 1.21.4.1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Acetoanaerobium sticklandii 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 10 * 31000, adjusted for the weight of water molecules lost as a result of peptide bond cleavage Acetoanaerobium sticklandii
298000
-
 sedimentation equilibrium centrifugation Acetoanaerobium sticklandii
327000
-
 gel filtration Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-proline + NADH Acetoanaerobium sticklandii NADH-dependent reduction of D-proline, NADH is the normal physiological electron donor, but the purified enzyme is inactive in presence of NADH in vitro 5-aminopentanoic acid + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.26
-
-
 Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-proline + dithiol (reduced)
-
 Acetoanaerobium sticklandii 5-aminopentanoic acid + oxidized dithiol
-
 ir
D-proline + dithiothreitol dithiothreitol as artificial electron donor Acetoanaerobium sticklandii 5-amino pentanoic acid + ?
-
 ir
D-proline + NADH NADH-dependent reduction of D-proline, NADH is the normal physiological electron donor, but the purified enzyme is inactive in presence of NADH in vitro Acetoanaerobium sticklandii 5-aminopentanoic acid + NAD+
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 30000-31000, SDS-PAGE Acetoanaerobium sticklandii
decamer 10 * 31000, adjusted for the weight of water molecules lost as a result of peptide bond cleavage Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
NADH normal physiological electron donor in vivo, highly specific, NADPH is inactive as electron donor Acetoanaerobium sticklandii