Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | activates oxidation of 2',4,4',6'-tetrahydroxychalcone | Antirrhinum majus |
Cloned (Comment) | Organism |
---|---|
gene AmAS1, DNA and amino acid sequence determination and analysis | Antirrhinum majus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits oxidation of 2',3,4,4',6'-pentahydroxychalcone | Antirrhinum majus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Antirrhinum majus | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | copper-containing enzyme, bound via histidine residues in the active site | Antirrhinum majus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is processed to the mature from a 65 kDa precursor protein by cleavage of the N-terminal part | Antirrhinum majus |
39000 | - |
1 * 39000, SDS-PAGE | Antirrhinum majus |
40000 | - |
mature enzyme, gel filtration | Antirrhinum majus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3,4,4',6'-pentahydroxychalcone + 1/2 O2 | Antirrhinum majus | - |
aureusidin + bracteatin + H2O | - |
ir | |
2',4,4',6'-tetrahydroxychalcone + O2 | Antirrhinum majus | - |
aureusidin + H2O | - |
ir | |
additional information | Antirrhinum majus | pathway, overview | ? | - |
? | |
additional information | Antirrhinum majus | enzyme plays a key role in the yellow coloration of flowers, enzyme is a homologue of plant polyphenol oxidase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Antirrhinum majus | - |
snapdragon | - |
Antirrhinum majus | - |
cv. Yellow Butterfly | - |
Antirrhinum majus | - |
gene AmAS1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Antirrhinum majus |
Purification (Comment) | Organism |
---|---|
107fold | Antirrhinum majus |
to homogeneity | Antirrhinum majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Antirrhinum majus | - |
additional information | analysis of spatial and temporal expression | Antirrhinum majus | - |
additional information | not in stem and leaf | Antirrhinum majus | - |
petal | enzyme expression | Antirrhinum majus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
578 | - |
purified enzyme | Antirrhinum majus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3,4,4',6'-pentahydroxychalcone + 1/2 O2 | - |
Antirrhinum majus | aureusidin + bracteatin + H2O | - |
ir | |
2',3,4,4',6'-pentahydroxychalcone + O2 | best substrate | Antirrhinum majus | aureusidin + bracteatin + H2O | products are formed in a 6:1 ratio | ir | |
2',3,4,4',6'-pentahydroxychalcone + O2 | 2210% activity compared to 2',4,4',6'-tetrahydroxychalcone | Antirrhinum majus | aureusidin + bracteatin + H2O | products are formed in a 6:1 ratio | ir | |
2',3,4,4',6'-pentahydroxychalcone 4'-beta-D-glucopyranoside + O2 | - |
Antirrhinum majus | aureusidin 6-beta-D-glucopyranoside + bracteatin 6-beta-D-glucopyranoside + H2O | - |
ir | |
2',4,4',6'-tetrahydroxychalcone + O2 | - |
Antirrhinum majus | aureusidin + H2O | - |
ir | |
2',4,4',6'-tetrahydroxychalcone 4'-beta-D-glucopyranoside + O2 | - |
Antirrhinum majus | aureusidin 6-beta-D-glucopyranoside + H2O | - |
ir | |
additional information | enzyme shows no 3',4'-dehydrogenase activity towards aureusidin | Antirrhinum majus | ? | - |
? | |
additional information | pathway, overview | Antirrhinum majus | ? | - |
? | |
additional information | enzyme plays a key role in the yellow coloration of flowers, enzyme is a homologue of plant polyphenol oxidase | Antirrhinum majus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Antirrhinum majus |
Synonyms | Comment | Organism |
---|---|---|
More | - |
- |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | broad, formation of aureusidin from 2',3,4,4',6'-pentahydroxychalcone | Antirrhinum majus |
5.4 | - |
3-hydroxylation and cyclization of 2',4,4',6'-tetrahydroxychalcone | Antirrhinum majus |