Crystallization (Comment) | Organism |
---|---|
crystal structure, molecular modeling of the active site structure and the Fe2+-binding motif | Streptomyces jumonjinensis |
Protein Variants | Comment | Organism |
---|---|---|
D214C | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
D214E | site-directed mutagenesis, active site mutant, retains 1% of activity compared to wild-type | Streptomyces jumonjinensis |
D214H | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H212D | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H212N | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H212Q | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H268D | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H268N | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
H268Q | site-directed mutagenesis, active site mutant, complete loss of activity | Streptomyces jumonjinensis |
additional information | exchange of Asp214 and His268, and exchange of Asp214 and His 212 by site-directed mutagenesis leads to completely inactive enzymes | Streptomyces jumonjinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-binding motif | Streptomyces jumonjinensis | |
Fe2+ | structure of the Fe2+ active site and endogenous ligands | Streptomyces jumonjinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces jumonjinensis | - |
isopenicillin N + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces jumonjinensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Streptomyces jumonjinensis | isopenicillin N + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Streptomyces jumonjinensis |