Activating Compound | Comment | Organism | Structure |
---|---|---|---|
O2 | required | Acremonium chrysogenum |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli, amino acid sequence comparison | Acremonium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
H116L | site-directed mutagenesis, reduced activity | Acremonium chrysogenum |
H126L | site-directed mutagenesis, reduced activity | Acremonium chrysogenum |
H137L | site-directed mutagenesis, reduced activity | Acremonium chrysogenum |
H262L | site-directed mutagenesis, complete loss of activity | Acremonium chrysogenum |
H272L | site-directed mutagenesis, complete loss of activity | Acremonium chrysogenum |
H49L | site-directed mutagenesis, complete loss of activity | Acremonium chrysogenum |
H64L | site-directed mutagenesis, reduced activity | Acremonium chrysogenum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Acremonium chrysogenum | |
Fe2+ | His216 and His 272 are ligands to bind the non-heme iron in the active site | Acremonium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, wild-type and mutants, SDS-PAGE | Acremonium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Acremonium chrysogenum | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants recombinant from Escherichia coli | Acremonium chrysogenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acremonium chrysogenum |
0.00000224 | - |
mutant H49L | Acremonium chrysogenum |
0.0000103 | - |
mutant H126L | Acremonium chrysogenum |
0.0000112 | - |
mutant H137L | Acremonium chrysogenum |
0.0000116 | - |
mutant H116L | Acremonium chrysogenum |
0.0000127 | - |
mutant H64L | Acremonium chrysogenum |
0.0000136 | - |
wild-type | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Acremonium chrysogenum | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Acremonium chrysogenum | isopenicillin N + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, wild-type and mutants, SDS-PAGE | Acremonium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Acremonium chrysogenum |