Literature summary for 1.20.99.1 extracted from

DeMel, S.; Shi, J.; Martin, P.; Rosen, B.P.; Edwards, B.F.
Arginine 60 in the ArsC arsenate reductase of E. coli plasmid R773 determines the chemical nature of the bound As(III) product (2004), Protein Sci., 13, 2330-2340.

Search for more literature for 1.20.99.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type and mutant R60K and R60A enzymes in complex with product arsenite, X-ray diffraction structure determination and analysis at 1.3-1.8 A resolution	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
R60A	site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity	Escherichia coli
R60K	site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
arsenate + reduced acceptor	Escherichia coli	detoxification enzyme, arsenite is excreted or sequestered	arsenite + acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene arsC, encoded on plasmid R773	-

Reaction

Reaction	Comment	Organism	Reaction ID
arsenite + acceptor + H2O = arsenate + reduced acceptor	the catalytic Cys12 of the wild-type enzyme, located at the active site and surrounded by the Arg triad with Arg60, Arg94, and Arg107, forms a unique monohydroxyl Cys12-thiol-arsenite adduct with a positive charge on the arsenic, which is an unstable intermediate	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + reduced acceptor	detoxification enzyme, arsenite is excreted or sequestered	Escherichia coli	arsenite + acceptor	-	?
arsenate + reduced acceptor	the chemical nature of As(III) product is determined by Arg60	Escherichia coli	arsenite + acceptor	-	?

Synonyms

Synonyms	Comment	Organism
ArsC	-	Escherichia coli
arsenate reductase	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)