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Literature summary for 1.20.2.1 extracted from

  • vanden Hoven, R.N.; Santini, J.M.
    Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite oxidase and its physiological electron acceptor (2004), Biochim. Biophys. Acta, 1656, 148-155.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
arsenite pH 5.5, temperature not specified in the publication Hydrogenophaga sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Hydrogenophaga sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains 10.6 mol/alpha3beta3 iron and 2.1 mol/alpha3beta3 molybdenum Hydrogenophaga sp.
Molybdenum protein contains 10.6 mol iron per alpha3beta3 unit and 2.1 mol molybdenum per alpha3beta3 molybdenum Hydrogenophaga sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE Hydrogenophaga sp.
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE Hydrogenophaga sp.
306000
-
gel filtration Hydrogenophaga sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arsenite + cytochrome c-551 + H2O Hydrogenophaga sp. the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth arsenate + reduced cytochrome c-551
-
?

Organism

Organism UniProt Comment Textmining
Hydrogenophaga sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hydrogenophaga sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
pH 5.5, temperature not specified in the publication Hydrogenophaga sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenite + cytochrome c-551 + H2O the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth Hydrogenophaga sp. arsenate + reduced cytochrome c-551
-
?
arsenite + H2O + 2,6-dichlorophenolindophenol
-
Hydrogenophaga sp. arsenate + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
hexamer 3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE Hydrogenophaga sp.

Synonyms

Synonyms Comment Organism
AroA
-
Hydrogenophaga sp.
aroB
-
Hydrogenophaga sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.4
-
arsenite pH 5.5, temperature not specified in the publication Hydrogenophaga sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Hydrogenophaga sp.

Cofactor

Cofactor Comment Organism Structure
cytochrome c-551 physiological electron acceptor for the arsenite oxidase Hydrogenophaga sp.