Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas stutzeri |
expression in Escherichia coli | Ralstonia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | 4 mM, 90% residual activity | Pseudomonas stutzeri | |
additional information | not inhibitory: arsenite, NaCl | Ralstonia sp. | |
NaCl | 4 mM, 87% residual activity | Pseudomonas stutzeri | |
nitrate | 4 mM, 21% residual activity | Pseudomonas stutzeri | |
nitrate | 4 mM, 40% residual activity | Ralstonia sp. | |
sulfite | 4 mM, 16% residual activity | Pseudomonas stutzeri | |
sulfite | 4 mM, 56% residual activity | Ralstonia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
NAD+ | pH 7.2, 30°C | Ralstonia sp. | |
0.0073 | - |
phosphonate | pH 7.2, 30°C | Pseudomonas stutzeri | |
0.0087 | - |
phosphonate | pH 7.2, 30°C | Ralstonia sp. | |
0.016 | - |
NAD+ | pH 7.2, 40°C | Ralstonia sp. | |
0.024 | - |
phosphonate | pH 7.2, 40°C | Ralstonia sp. | |
0.056 | - |
NAD+ | pH 7.2, 30°C | Pseudomonas stutzeri | |
0.128 | - |
NAD+ | pH 7.2, 40°C | Pseudomonas stutzeri | |
0.287 | - |
phosphonate | pH 7.2, 40°C | Pseudomonas stutzeri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant protein | Ralstonia sp. | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Pseudomonas stutzeri WM88 | - |
- |
- |
Ralstonia sp. | G4XDR8 | - |
- |
Ralstonia sp. 4506 | G4XDR8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Ralstonia sp. | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri WM88 | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Ralstonia sp. 4506 | phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PtxD | - |
Pseudomonas stutzeri |
PtxD | - |
Ralstonia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas stutzeri |
50 | - |
- |
Ralstonia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life 80.5 h | Pseudomonas stutzeri |
45 | - |
half-life 80.5 h | Ralstonia sp. |