Application | Comment | Organism |
---|---|---|
biotechnology | improvement of enzyme for use in regeneration of NADH and NADPH, application in bioconversion of trimethylpyruvate to L-tert-leucine as model reaction | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
D13E/M26I/E175A/E332N/C336D | mutant obtained by directed evolution, round 4 | Pseudomonas stutzeri |
D13E/M26I/E175A/T181S/A308T/E332N/C336D | mutant obtained by directed evolution, round 6, strong decrease in KM value for NADP compared to wild-type | Pseudomonas stutzeri |
D13E/M26I/E175A/T181S/E332N/C336D | mutant obtained by directed evolution, round 5 | Pseudomonas stutzeri |
D13E/M26I/E332N/C336D | mutant obtained by directed evolution, round 3 | Pseudomonas stutzeri |
E175A | mutant with significantly increased kcat value for NADP+ | Pseudomonas stutzeri |
K330* | mutant obtained by directed evolution, round 1 | Pseudomonas stutzeri |
M26I/E332N/C336D | mutant obtained by directed evolution, round 2 | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
phosphite | cosubstrate NAD+, mutant K330*, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.027 | - |
phosphite | cosubstrate NAD+, mutant M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.03 | - |
phosphite | cosubstrate NAD+, mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.032 | - |
NAD+ | mutant M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.034 | - |
NAD+ | mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.037 | - |
NAD+ | mutant K330*, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.039 | - |
NAD+ | mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.041 | - |
NAD+ | mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.045 | - |
NAD+ | mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.047 | - |
phosphite | cosubstrate NAD+, wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.048 | - |
phosphite | cosubstrate NAD+, mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.053 | - |
NAD+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.056 | - |
NADP+ | mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.057 | - |
NADP+ | mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.062 | - |
phosphite | cosubstrate NADP+, mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.065 | - |
NADP+ | mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.07 | - |
phosphite | cosubstrate NADP+, mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.082 | - |
phosphite | cosubstrate NAD+, mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.084 | - |
phosphite | cosubstrate NAD+, mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.087 | - |
phosphite | cosubstrate NADP+, mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.138 | - |
phosphite | cosubstrate NADP+, mutant E175A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.14 | - |
NADP+ | mutant E175A, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.07 | - |
NADP+ | mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.9 | - |
phosphite | cosubstrate NADP+, mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.9 | - |
phosphite | cosubstrate NADP+, wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.5 | - |
NADP+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphite + H2O + NAD+ | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? | |
phosphite + H2O + NADP+ | - |
Pseudomonas stutzeri | phosphate + NADPH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
NADP+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.75 | - |
NADP+ | mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.8 | - |
NADP+ | mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.8 | - |
NADP+ | mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.9 | - |
NADP+ | mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.2 | - |
NADP+ | mutant E175A, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.8 | - |
NAD+ | mutant K330*, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.9 | - |
NAD+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.6 | - |
NAD+ | mutant M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.7 | - |
NAD+ | mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
4 | - |
NAD+ | mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.3 | - |
NAD+ | mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri | |
5.7 | - |
NAD+ | mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C | Pseudomonas stutzeri |