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Literature summary for 1.20.1.1 extracted from

  • Johannes, T.W.; Woodyer, R.D.; Zhao, H.
    Efficient regeneration of NADPH using an engineered phosphite dehydrogenase (2007), Biotechnol. Bioeng., 96, 18-26.
    View publication on PubMed

Application

Application Comment Organism
biotechnology application of mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V/A176R for regeneration of NADPH in xylose reductase-catalyzed xylitol synthesis and alcohol dehydrogenase-catalyzed (R)-phenylethanol synthesis. comparison of enzyme with commercial Pseudomonas sp. formate dehydrogenase. Mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V/A176R shows higher substrate conversion and higher total turnover numbers for NADP+ than formate dehydrogenase Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V/A176R highly stable and active mutant engineered for regeneration of NADPH and enzyme membrane reactors Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
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