Application | Comment | Organism |
---|---|---|
biotechnology | evaluation of enzyme mutants for regeneration of reduced cofactors NADH and NADPH in industrial processes and comparison with Candida boidinii formate dehydrogenase | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
I150F | thermostable mutant, half-life at 45°C 7.0 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q132R | thermostable mutant, half-life at 45°C 2.3 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q132R/Q137R/I150F/Q215L/R275Q | thermostable mutant, half-life at 45°C 161 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R | thermostable mutant, half-life at 45°C 3.8 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q | thermostable mutant, half-life at 45°C 200 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/A319E | thermostable mutant, half-life at 45°C 567 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/D162N/V315A | thermostable mutant, half-life at 45°C 614 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q | thermostable mutant, half-life at 45°C 437 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A | thermostable mutant, half-life at 45°C 1421 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V | thermostable mutant, half-life at 45°C 2315 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K | thermostable mutant, half-life at 45°C 1515 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L | thermostable mutant, half-life at 45°C 1765 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R | thermostable mutant, half-life at 45°C 2350 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V | thermostable mutant, half-life at 45°C 8440 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I | thermostable mutant, half-life at 45°C 2000 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
Q215L | thermostable mutant, half-life at 45°C 8.7 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
R275Q | thermostable mutant, half-life at 45°C 4.6 min compared to 1.4 min of wild-type | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.045 | - |
phosphite | mutant Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.046 | - |
NAD+ | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.046 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.048 | - |
phosphite | mutant Q137R, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.05 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.052 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.056 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.057 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.057 | - |
phosphite | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.058 | - |
phosphite | mutant Q215L, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.06 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.063 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.064 | - |
NAD+ | mutant Q215L, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.065 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.066 | - |
NAD+ | mutant Q137R, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.066 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.066 | - |
NAD+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.07 | - |
NAD+ | mutant R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.071 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.074 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.075 | - |
NAD+ | mutant I150F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.075 | - |
phosphite | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.075 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.078 | - |
phosphite | mutant R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.092 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.096 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.099 | - |
phosphite | mutant I150F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.105 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.12 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.123 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.135 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.144 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.155 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.156 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.169 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphite + H2O + NAD+ | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
wild-type, melting temperature | Pseudomonas stutzeri |
59.3 | - |
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, melting temperature | Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
NAD+ | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.85 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.9 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.9 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.13 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.6 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.96 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.1 | - |
NAD+ | mutant R275Q, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.4 | - |
NAD+ | mutant I150F, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.4 | - |
NAD+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.6 | - |
NAD+ | mutant Q215L, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.75 | - |
NAD+ | mutant Q137R, pH 7.3, 25°C | Pseudomonas stutzeri |