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Literature summary for 1.2.98.1 extracted from
- Formaldehyde dismutase, a novel NAD-binding oxidoreductase from Pseudomonas putida F61 (1986), Eur. J. Biochem., 156, 59-64.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Pseudomonas putida F61 | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Pseudomonas putida |  |
| additional information | not inhibited by AMP, ADP, ATP and N-methylnicotinamide chloride | Pseudomonas putida | |
| pyrazole | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
1-butanol | + p-nitroso-N,N-dimethylaniline | Pseudomonas putida | |
| 0.0032 | - |
1-propanol | + p-nitroso-N,N-dimethylaniline | Pseudomonas putida | |
| 0.02 | - |
ethanol | + p-nitroso-N,N-dimethylaniline | Pseudomonas putida | |
| 0.04 | - |
methanol | + p-nitroso-N,N-dimethylaniline | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | each subunit of the tetramer contains 2 mol zinc per mol | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, SDS-PAGE | Pseudomonas putida |
| 180000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
F61 | - |
| Pseudomonas putida F61 | - |
F61 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-butanol + p-nitroso-N,N-dimethylaniline | 96.2% of activity with ethanol or methanol | Pseudomonas putida | n-butanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| 1-butanol + p-nitroso-N,N-dimethylaniline | 96.2% of activity with ethanol or methanol | Pseudomonas putida F61 | n-butanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| 1-pentanol + p-nitroso-N,N-dimethylaniline | 9.62% of activity with ethanol | Pseudomonas putida | n-pentanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| 1-pentanol + p-nitroso-N,N-dimethylaniline | 9.62% of activity with ethanol | Pseudomonas putida F61 | n-pentanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| 1-propanol + p-nitroso-N,N-dimethylaniline | - |
Pseudomonas putida | n-propanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| 1-propanol + p-nitroso-N,N-dimethylaniline | - |
Pseudomonas putida F61 | n-propanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| ethanol + p-nitroso-N,N-dimethylaniline | - |
Pseudomonas putida | ethanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| ethanol + p-nitroso-N,N-dimethylaniline | - |
Pseudomonas putida F61 | ethanal + reduced p-nitroso-N,N-dimethylaniline | - |
? | |
| formaldehyde + H2O | dismutation | Pseudomonas putida | formate + methanol | - |
? | |
| formaldehyde + H2O | dismutation | Pseudomonas putida F61 | formate + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, SDS-PAGE | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | each subunit contains 1 mol NADH per mol, binds firmly at each active site, enzyme-bound NAD(H) is reduced and oxidized only by the added second substrate (alcohol, aldehyde) not by exogenous electron acceptor | Pseudomonas putida | |
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