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Literature summary for 1.2.7.B2 extracted from

  • Mukund, S.; Adams, M.W.
    Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism (1993), J. Biol. Chem., 268, 13592-13600.
    View publication on PubMed
Search for more literature for 1.2.7.B2

Inhibitors

Inhibitors Comment Organism Structure
arsenite 5 mM, 50% inhibition after min Thermococcus litoralis
cyanide 5 mM, 50% inhibition after min Thermococcus litoralis
iodoacetate 5 mM, 50% inhibition after min Thermococcus litoralis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states Thermococcus litoralis
Tungsten tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Thermococcus litoralis
70000
-
 4 * 70000, SDS-PAGE Thermococcus litoralis

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis Q56303
-
-
Thermococcus litoralis DSM 5473 Q56303
-
-

Oxidation Stability

Oxidation Stability Organism
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h Thermococcus litoralis

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.8
-
 pH 8.4, 80°C Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + oxidized methyl viologen
-
 Thermococcus litoralis acetate + H+ + reduced methyl viologen
-
 ?
acetaldehyde + H2O + oxidized methyl viologen
-
 Thermococcus litoralis DSM 5473 acetate + H+ + reduced methyl viologen
-
 ?
butyraldehyde + H2O + oxidized methyl viologen 10% compared to the activity with acetyladehyde Thermococcus litoralis butyrate + H+ + reduced methyl viologen
-
 ?
butyraldehyde + H2O + oxidized methyl viologen 10% compared to the activity with acetyladehyde Thermococcus litoralis DSM 5473 butyrate + H+ + reduced methyl viologen
-
 ?
formaldehyde + H2O + 2 oxidized ferredoxin
-
 Thermococcus litoralis formate + 2 H+ + 2 reduced ferredoxin
-
 ?
formaldehyde + H2O + 2 oxidized ferredoxin
-
 Thermococcus litoralis DSM 5473 formate + 2 H+ + 2 reduced ferredoxin
-
 ?
formaldehyde + H2O + oxidized methyl viologen 63% compared to the activity with acetyladehyde Thermococcus litoralis formate + H+ + reduced methyl viologen
-
 ?
formaldehyde + H2O + oxidized methyl viologen 63% compared to the activity with acetyladehyde Thermococcus litoralis DSM 5473 formate + H+ + reduced methyl viologen
-
 ?
additional information activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde Thermococcus litoralis ?
-
 ?
additional information activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde Thermococcus litoralis DSM 5473 ?
-
 ?
propionaldehyde + H2O + oxidized methyl viologen 77% compared to the activity with acetyladehyde Thermococcus litoralis propionate + H+ + reduced methyl viologen
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 70000, SDS-PAGE Thermococcus litoralis

Synonyms

Synonyms Comment Organism
FOR
-
 Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 2 h, 50% loss of activity Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Thermococcus litoralis