Activating Compound | Comment | Organism | Structure |
---|---|---|---|
maltose | stimulates about 4fold | Pyrobaculum aerophilum |
Application | Comment | Organism |
---|---|---|
degradation | Pyrobaculum aerophilum contains a modified Embden-Meyerhof pathway, in which GAPOR replaces the GAPDH/phosphoglycerate kinase couple of the conventional EmbdenMeyerhof pathway | Pyrobaculum aerophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
D-Glyceraldehyde-3-phosphate | - |
Pyrobaculum aerophilum | |
0.043 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 50°C | Pyrobaculum aerophilum | |
1 | - |
benzyl viologen | - |
Pyrobaculum aerophilum | |
1 | - |
Oxidized benzyl viologen | pH 7.8, 50°C | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Pyrobaculum aerophilum |
65000 | - |
1 * 65000, SDS-PAGE | Pyrobaculum aerophilum |
66000 | - |
1 * 66000, SDS-PAGE | Pyrobaculum aerophilum |
70000 | - |
PAE1029, represents the GAPOR encoding gene gapor, sequence analysis | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Aeropyrum pernix | - |
- |
- |
Pyrobaculum aerophilum | - |
- |
- |
Pyrobaculum aerophilum | Q8ZXY6 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZXY6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
by gel filtration, 113fold, to homogeneity | Pyrobaculum aerophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
cell free extract, at 50°C | Pyrobaculum aerophilum |
0.375 | - |
at 50°C | Pyrobaculum aerophilum |
17 | - |
113fold purified, at 50°C, with a yield of 0.2% | Pyrobaculum aerophilum |
17 | - |
pH 7.8, 50°C | Pyrobaculum aerophilum |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 9, several weeks | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen | - |
Pyrobaculum aerophilum | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen | - |
Pyrobaculum aerophilum DSM 7523 | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen | is highly specific for glyceraldehyde-3-phosphate | Pyrobaculum aerophilum | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
additional information | does not use glyceraldehyde, acetaldehyde or formaldehyde as substrates | Pyrobaculum aerophilum | ? | - |
? | |
additional information | enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde | Pyrobaculum aerophilum | ? | - |
? | |
additional information | enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde | Pyrobaculum aerophilum DSM 7523 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Pyrobaculum aerophilum |
monomer | 1 * 66000, SDS-PAGE | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
aldehyde ferredoxin oxidoreductase | - |
Pyrobaculum aerophilum |
GAPOR | - |
Pyrobaculum aerophilum |
glyceraldehyde-3-phosphate ferredoxin oxidoreductase | - |
Pyrobaculum aerophilum |
PAE1029 | - |
Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
retains 70% of its activity upon incubation for 2 h (half-life, 500 min) | Pyrobaculum aerophilum |
100 | - |
2 h, 70% residual activity | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Pyrobaculum aerophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
60% of maximum activity | Pyrobaculum aerophilum |
8.3 | - |
60% of maximum activity | Pyrobaculum aerophilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.7 | 8.3 | 60% activity at pH 7.7 and 8.3 | Pyrobaculum aerophilum |
General Information | Comment | Organism |
---|---|---|
metabolism | extracts of maltose/yeast extract/nitrate-grown cells contain all enzyme activities of a modified Embden-Meyerhof pathway, including ATP-dependent glucokinase, phosphoglucose isomerase, ATP-dependent 6-phosphofructokinase, fructose-1,6-phosphate aldolase, triose-phosphate isomerase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, phosphoglycerate mutase, enolase and pyruvate kinase | Pyrobaculum aerophilum |