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Literature summary for 1.2.7.4 extracted from

  • Terlesky, K.C.; Nelson, M.J.; Ferry, J.G.
    Isolation of an enzyme complex with carbon monoxide dehydrogenase activity containing corrinoid and nickel from acetate-grown Methanosarcina thermophila (1986), J. Bacteriol., 168, 1053-1058.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
corrinoid cofactor the multifunctional enzyme complex contains a corrinoid cofactor Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
KCN
-
Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the multifunctional enzyme complex contains Co2+ Methanosarcina thermophila
Fe-S center the multifunctional enzyme complex contains iron-sulfur centers Methanosarcina thermophila
Ni2+ the multifunctional enzyme complex contains Ni2+ Methanosarcina thermophila
Zn2+ the multifunctional enzyme complex contains Zn2+ Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
58000
-
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
60000
-
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
71000
-
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
89000
-
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
250000
-
the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis Methanosarcina thermophila
1000000
-
the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-
Methanosarcina thermophila TM-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells Methanosarcina thermophila
-
culture condition:methanol-grown cell activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells Methanosarcina thermophila
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + FAD at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila CO2 + FADH2
-
?
CO + H2O + FAD at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila TM-1 CO2 + FADH2
-
?
CO + H2O + FMN at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila CO2 + FMNH2
-
?
CO + H2O + FMN at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila TM-1 CO2 + FMNH2
-
?
CO + H2O + oxidized methyl viologen no enzyme with cofactor F420 Methanosarcina thermophila CO2 + reduced methyl viologen
-
?
CO + H2O + oxidized methyl viologen no enzyme with cofactor F420 Methanosarcina thermophila TM-1 CO2 + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
? x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila

Synonyms

Synonyms Comment Organism
CO dehydrogenase
-
Methanosarcina thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
assay with methyl viologen Methanosarcina thermophila