Activating Compound | Comment | Organism | Structure |
---|---|---|---|
corrinoid cofactor | the multifunctional enzyme complex contains a corrinoid cofactor | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | - |
Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the multifunctional enzyme complex contains Co2+ | Methanosarcina thermophila | |
Fe-S center | the multifunctional enzyme complex contains iron-sulfur centers | Methanosarcina thermophila | |
Ni2+ | the multifunctional enzyme complex contains Ni2+ | Methanosarcina thermophila | |
Zn2+ | the multifunctional enzyme complex contains Zn2+ | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
58000 | - |
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
60000 | - |
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
71000 | - |
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
89000 | - |
x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
250000 | - |
the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis | Methanosarcina thermophila |
1000000 | - |
the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Methanosarcina thermophila TM-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells | Methanosarcina thermophila | - |
culture condition:methanol-grown cell | activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells | Methanosarcina thermophila | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + FAD | at 15% of the specific activity compared to methyl viologen | Methanosarcina thermophila | CO2 + FADH2 | - |
? | |
CO + H2O + FAD | at 15% of the specific activity compared to methyl viologen | Methanosarcina thermophila TM-1 | CO2 + FADH2 | - |
? | |
CO + H2O + FMN | at 15% of the specific activity compared to methyl viologen | Methanosarcina thermophila | CO2 + FMNH2 | - |
? | |
CO + H2O + FMN | at 15% of the specific activity compared to methyl viologen | Methanosarcina thermophila TM-1 | CO2 + FMNH2 | - |
? | |
CO + H2O + oxidized methyl viologen | no enzyme with cofactor F420 | Methanosarcina thermophila | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + oxidized methyl viologen | no enzyme with cofactor F420 | Methanosarcina thermophila TM-1 | CO2 + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE | Methanosarcina thermophila |
Synonyms | Comment | Organism |
---|---|---|
CO dehydrogenase | - |
Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Methanosarcina thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay with methyl viologen | Methanosarcina thermophila |