Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | 0.075 mM, reversible, competitive inhibition of reduced CODHII with respect to the substrate carbon monoxide, which protects reduced CODHII against inhibition by cyanide, inhibited CODHII regains initial activity after a 15-25-min incubation at 70°C with dithionite or Ti(III) citrate under CO or N2, while slower and partial reactivation to 30-50% of the initial activity occurs with dithiothreitol or without reductants | Carboxydothermus hydrogenoformans | |
potassium cyanide | competitive inhibitor of reduced CODHII with respect to the substrate CO, inhibition of dithionite- or Ti(III) citrate-reduced CODHII by potassium cyanide is fully reversible since the enzyme can be completely reactivated, sodium sulfide has no effect on the reactivation of cyanide-inhibited CODHII in the presence of dithionite | Carboxydothermus hydrogenoformans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains one Ni-4Fe-5S cluster | Carboxydothermus hydrogenoformans | |
Ni2+ | contains one Ni-4Fe-5S cluster | Carboxydothermus hydrogenoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | - |
strain Z-2901 (DSM 6008) | - |
Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 | - |
strain Z-2901 (DSM 6008) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + methyl viologen | - |
Carboxydothermus hydrogenoformans | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + methyl viologen | - |
Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 | CO2 + reduced methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CO dehydrogenase | - |
Carboxydothermus hydrogenoformans |
CODHII | - |
Carboxydothermus hydrogenoformans |
Ni-Fe carbon monoxide dehydrogenase II | - |
Carboxydothermus hydrogenoformans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ni-4Fe-5S center | active site cluster | Carboxydothermus hydrogenoformans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0217 | - |
cyanide | at 23°C, in 4 mM dithionite, and 4 mM dithiothreitol | Carboxydothermus hydrogenoformans |