Protein Variants | Comment | Organism |
---|---|---|
A219F | mutant designed to block tunnel between Ni-Fe-S active site clusters, little enzymic activity. Metal clusters are properly assembled, impaired ability of CO to migrate through the tunnel | Moorella thermoacetica |
A578C | mutant designed to block tunnel between Ni-Fe-S active site clusters, little enzymic activity. Metal clusters are properly assembled, impaired ability of CO to migrate through the tunnel | Moorella thermoacetica |
F70W | mutant designed to block region that connects the CO tunnel at the betabeta interface with a water channel, little enzymic activity. Metal clusters are properly assembled, impaired ability of CO to migrate through the tunnel | Moorella thermoacetica |
L215F | mutant designed to block tunnel between Ni-Fe-S active site clusters, little enzymic activity. Metal clusters are properly assembled, impaired ability of CO to migrate through the tunnel | Moorella thermoacetica |
N101Q | mutant designed to block region that connects the CO tunnel at the betabeta interface with a water channel, little enzymic activity. Metal clusters are properly assembled, impaired ability of CO to migrate through the tunnel | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CO + H2O + 2 oxidized ferredoxin = CO2 + 2 reduced ferredoxin + 2 H+ | CO and CO2 enter and exit the enzyme at the water channel along the betabeta subunit interface. CO either enters the enzyme and migrates through the tunnel before binding at the A-cluster, or it binds the A-cluster directly from solvent | Moorella thermoacetica |