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Literature summary for 1.2.7.4 extracted from

  • Tan, X.; Loke, H.K.; Fitch, S.; Lindahl, P.A.
    The tunnel of acetyl-coenzyme A synthase/carbon monoxide dehydrogenase regulates delivery of CO to the active site (2005), J. Am. Chem. Soc., 127, 5833-5839.
    View publication on PubMed
Search for more literature for 1.2.7.4

Activating Compound

Activating Compound Comment Organism Structure
Nickel Ni-activated alpha subunit rapidly and reversibly accepts a methyl group Moorella thermoacetica

Protein Variants

Protein Variants Comment Organism
A110C mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, no synthesis of acetyl-CoA Moorella thermoacetica
A222L mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, no synthesis of acetyl-CoA Moorella thermoacetica
A265M mutation within tunnel region of alpha subunit, absence of strong cooperative inhibition of CO, little synthesis of acetyl-CoA Moorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
CO weak, isolated alpha subunit Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 CO 25°C, isolated alpha subunit Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
 bifunctional acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, isolated alpha subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 25°C, mutant A265M Moorella thermoacetica
200
-
 25°C, wild type Moorella thermoacetica
280
-
 25°C, mutant A110C Moorella thermoacetica
300
-
 25°C, mutant A222L Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + acceptor
-
 Moorella thermoacetica CO2 + reduced acceptor
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 CO 25°C, isolated alpha subunit Moorella thermoacetica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
 CO 25°C Moorella thermoacetica