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Literature summary for 1.2.7.4 extracted from

  • Ragsdale, S.W.; Clark, J.E.; Ljungdahl, L.G.; Lundie, L.L.; Drake, H.L.
    Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein (1983), J. Biol. Chem., 258, 2364-2369.
    View publication on PubMed
Search for more literature for 1.2.7.4

General Stability

General Stability Organism
98% loss of activity after 15 min in an aerobic buffer Moorella thermoacetica
the presence of CO has no apparent effect on the stability Moorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.04 mM, 60% inhibition within 10 s, CO reverses inhibition Moorella thermoacetica
KCN CO reverses cyanide inhibition, but promotes reaction with methyl iodide Moorella thermoacetica
methyl iodide CO promotes inhibition; irreversible inhibition Moorella thermoacetica
additional information pyruvate and ATP have no effect Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.03
-
 methyl viologen 50°C, pH 8.4 Moorella thermoacetica
3.03
-
 methyl viologen pH 8.4, 50°C Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Moorella thermoacetica
Iron contains 11 mol of iron and 14 mol of acid-labile sulfur per mol of alphabeta dimer Moorella thermoacetica
Ni2+
-
 Moorella thermoacetica
Nickel contains 2 mol of nickel per mol of alphabeta dimer Moorella thermoacetica
Zinc contains 1 mol of zinc per mol of alphabeta dimer Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE Moorella thermoacetica
71000
-
 3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE Moorella thermoacetica
78000
-
 3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE Moorella thermoacetica
78000
-
 3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE Moorella thermoacetica
155000
-
 sedimentation equilibrium centrifugation Moorella thermoacetica
161000
-
 pore limit gel electrophoresis Moorella thermoacetica
436000
-
 gel filtration Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO + H2O + oxidized ferredoxin Moorella thermoacetica initial step of CO metabolism in acetogenic bacteria CO2 + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-
Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-
Moorella thermoacetica
-
 ATCC 39073
-

Oxidation Stability

Oxidation Stability Organism
addition of the enzyme to an aerobic buffer, 50 mM Tris/HCl, pH 7.6 results in a 98% loss of activity within 15 min, presence of CO has no apparent effect on the stability of the enzyme Moorella thermoacetica
extreme oxygen lability Moorella thermoacetica

Purification (Commentary)

Purification (Comment) Organism
-
 Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
675
-
 electron acceptor methyl viologen Moorella thermoacetica

Storage Stability

Storage Stability Organism
-20°C or 5°C, 50 mM Tris/HCl, pH 7.5, 2 mM sodium dithionite, 0.2 mM methyl viologen, 50% v/v glycerol, stable for more than 1 month Moorella thermoacetica
-20°C, stored frozen in an oxygen-free atmosphere in buffer containing glycerol and 2 mM dithionite, not stable for more than 1 month Moorella thermoacetica
10°C, Tris/HCl, pH 7.6, enzyme is active for at least 2 days Moorella thermoacetica
5°C, stored in an oxygen-free atmosphere in buffer containing glycerol and 2 mM dithionite, not stable for more than 1 month Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + ferredoxin
-
 Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + flavodoxin
-
 Moorella thermoacetica CO2 + reduced flavodoxin
-
 ?
CO + H2O + FMN
-
 Moorella thermoacetica CO2 + FMNH2
-
 ?
CO + H2O + methyl viologen
-
 Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
CO + H2O + methylene blue
-
 Moorella thermoacetica CO2 + reduced methylene blue
-
 ?
CO + H2O + oxidized ferredoxin ferredoxin I and II Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + oxidized ferredoxin initial step of CO metabolism in acetogenic bacteria Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + oxidized flavodoxin
-
 Moorella thermoacetica CO2 + reduced flavodoxin
-
 ?
CO + H2O + oxidized methyl viologen
-
 Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
CO + H2O + oxidized methylene blue
-
 Moorella thermoacetica CO2 + reduced methylene blue
-
 ?
CO + H2O + oxidized rubredoxin most efficient electron acceptor Moorella thermoacetica CO2 + reduced rubredoxin
-
 ?
CO + H2O + oxidized viologen
-
 Moorella thermoacetica CO2 + reduced viologen
-
 ?
CO + H2O + rubredoxin most efficient electron acceptor Moorella thermoacetica CO2 + reduced rubredoxin
-
 ?
additional information pure enzyme has no hydrogenase or formate dehydrogenase activity Moorella thermoacetica ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE Moorella thermoacetica
hexamer 3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE Moorella thermoacetica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 70 purification can be done at 25°C without loss of activity, heating for 30 min at 67°C leads to apparent loss, heating for only a few min at 70°C activates the enzyme Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.4
-
 Moorella thermoacetica