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Literature summary for 1.2.7.4 extracted from

  • DeMoll, E.; Grahame, D.A.; Harnly, J.M.; Tsai, L.; Stadtman, T.C.
    Purification and properties of carbon monoxide dehydrogenase from Methanococcus vannielii (1987), J. Bacteriol., 169, 3916-3920.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.4

Metals/Ions

Metals/Ions Comment Organism Structure
Nickel contains 2 g-atoms of nickel per mol of enzyme Methanococcus vannielii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 2 * 21000 + 2 * 89000, SDS-PAGE Methanococcus vannielii
89000
-
 2 * 21000 + 2 * 89000, SDS-PAGE Methanococcus vannielii
220000
-
 nondenaturing PAGE Methanococcus vannielii

Organism

Organism UniProt Comment Textmining
Methanococcus vannielii
-
-
-

Oxidation Stability

Oxidation Stability Organism
extremely oxygen-labile, even in N2 atmosphere with less than 5 ppm O2 the pure enzyme loses a significant amount of its activity within 18 h, 2 mM dithionite protects from inactivation Methanococcus vannielii

Purification (Commentary)

Purification (Comment) Organism
-
 Methanococcus vannielii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ammonium malate-grown cell
-
 Methanococcus vannielii
-
culture condition:formate-grown cell
-
 Methanococcus vannielii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
-
 Methanococcus vannielii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + 1,1'-trimethylene-4,4'-dimethyl-2,2'dipyridylium dibromide
-
 Methanococcus vannielii CO2 + reduced 1,1'-trimethylene-4,4'-dimethyl-2,2'dipyridylium dibromide
-
 ?
CO + H2O + 2,3,5-triphenyltetrazolium chloride
-
 Methanococcus vannielii CO2 + reduced 2,3,5-triphenyltetrazolium chloride
-
 ?
CO + H2O + FAD
-
 Methanococcus vannielii CO2 + FADH2
-
 ?
CO + H2O + ferredoxin
-
 Methanococcus vannielii CO2 + reduced ferredoxin
-
 ?
CO + H2O + FMN
-
 Methanococcus vannielii CO2 + FMNH2
-
 ?
CO + H2O + methyl viologen no activity with NAD+ and NADP+ Methanococcus vannielii CO2 + reduced methyl viologen
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 21000 + 2 * 89000, SDS-PAGE Methanococcus vannielii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Methanococcus vannielii

pH Range

pH Minimum pH Maximum Comment Organism
8 11.5 pH 8: about 55% of maximal activity, pH 11.5: about 50% of maximal activity Methanococcus vannielii