General Stability | Organism |
---|---|
CO renders the enzyme more susceptible to temperature inactivation | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | - |
Methanosarcina barkeri | |
CN- | - |
Methanosarcina barkeri | |
Glyoxaldehyde | inactivation requires enzymatic turnover; only with CO and methyl viologen as substrates | Methanosarcina barkeri | |
KCN | - |
Methanosarcina barkeri | |
additional information | dimethylglyoxime is no inhibitor, formaldehyde and acetaldehyde does not inactivate the enzyme; not inhibited by 20 mM formaldehyde or acetaldehyde | Methanosarcina barkeri | |
O2 | - |
Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
2,3,5-triphenyltetrazolium chloride | below 0.5 mM | Methanosarcina barkeri | |
0.5 | - |
2,3,5-triphenyltetrazolium chloride | pH 7.0, 25°C | Methanosarcina barkeri | |
7.1 | - |
methyl viologen | - |
Methanosarcina barkeri | |
7.1 | - |
methyl viologen | pH 7.0, 25°C | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | tightly bound by the enzyme | Methanosarcina barkeri | |
Iron | contains 15.6 mol of iron per mol of alpha2beta oligomer | Methanosarcina barkeri | |
additional information | no stimulation by MgSO4, CoCl2, NiCl2 and ZnSO4 | Methanosarcina barkeri | |
Ni2+ | tightly bound by the enzyme | Methanosarcina barkeri | |
Nickel | contains 1.3 mol of nickel per mol of alpha2beta2 oligomer | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19700 | - |
2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
19700 | - |
2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
84500 | - |
2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
84500 | - |
2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
161000 | - |
gel filtration | Methanosarcina barkeri |
161000 | - |
dialyzed enzyme, gel filtration | Methanosarcina barkeri |
205000 | - |
gradient gel electrophoresis | Methanosarcina barkeri |
3000000 | - |
high molecular weight form exists under conditions of high ionic strength, gel filtration | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + oxidized ferredoxin | Methanosarcina barkeri | may participate in methanogenesis by cleavage of acetate, reverse of the reaction in acetate biosynthesis | CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
extremely sensitive to air, most of the enzyme activity is lost upon exposure to air for 1 min, longer incubation results in complete inactivation | Methanosarcina barkeri |
strong inhibition by oxygen, extremely sensitive, exposed to air activity is lost in less than 1 min | Methanosarcina barkeri |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Methanosarcina barkeri | - |
culture condition:ammonium malate-grown cell | - |
Methanosarcina barkeri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133.6 | - |
- |
Methanosarcina barkeri |
Storage Stability | Organism |
---|---|
-70°C, samples thawed under anaerobic conditions exhibits full activity even after 6 months of storage | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + 2,3,5-triphenyltetrazolium chloride | - |
Methanosarcina barkeri | CO2 + reduced 2,3,5-triphenyltetrazolium chloride | - |
? | |
CO + H2O + FAD | - |
Methanosarcina barkeri | CO2 + FADH2 | - |
? | |
CO + H2O + ferredoxin | - |
Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + FMN | - |
Methanosarcina barkeri | CO2 + FMNH2 | - |
? | |
CO + H2O + methyl viologen | the enzyme fails to reduce NAD+, NADP+, or 8-hydroxy-5-deazaflavin cofactor 420 | Methanosarcina barkeri | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + oxidized 2,3,5-triphenyltetrazolium chloride | - |
Methanosarcina barkeri | CO2 + reduced 2,3,5-triphenyltetrazolium chloride | - |
? | |
CO + H2O + oxidized ferredoxin | - |
Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized ferredoxin | may participate in methanogenesis by cleavage of acetate, reverse of the reaction in acetate biosynthesis | Methanosarcina barkeri | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized flavodoxin | - |
Methanosarcina barkeri | CO2 + reduced flavodoxin | - |
? | |
CO + H2O + oxidized methyl viologen | - |
Methanosarcina barkeri | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + oxidized phenazine methosulfate | - |
Methanosarcina barkeri | CO2 + reduced phenazine methosulfate | - |
? | |
CO + H2O + phenazine methosulfate | - |
Methanosarcina barkeri | CO2 + reduced phenazine methosulfate | - |
? | |
additional information | enzyme fails to reduce NAD+, NADP+ or the 8-hydroxy-5-deazaflavin factor F420 | Methanosarcina barkeri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 84500 + 2 * 19700, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
tetramer | 2 * 19700 + 2 * 84500, alpha2beta2, SDS-PAGE | Methanosarcina barkeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
CO renders the enzyme more susceptible to temperature inactivation | Methanosarcina barkeri |
24 | - |
10 min, about 30% loss of activity in presence of CO, stable in absence of CO | Methanosarcina barkeri |
39 | - |
10 min, 45% loss of activity in presence of CO, stable in absence of CO | Methanosarcina barkeri |
62 | - |
10 min, about 65% loss of activity in presence of CO, about 5% loss of activity in absence of CO | Methanosarcina barkeri |
80 | - |
10 min, about 85% loss of activity in presence of CO, about 20% loss of activity in absence of CO | Methanosarcina barkeri |
80 | 104 | most of the activity remains after heating at 80°C, nearly complete inactivation after heating at 104°C, greater loss of activity at all temperatures when heated in presence of CO | Methanosarcina barkeri |
104 | - |
10 min, complete loss of activity in presence or in absence of CO | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Methanosarcina barkeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | very little activity outside this range | Methanosarcina barkeri |