Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated | Methanosarcina barkeri | |
CN- | molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated | Methanothermobacter thermautotrophicus | |
CN- | molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated | Methanothermobacter wolfeii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
N-formylmethanofuran | molybdenum enzyme | Methanothermobacter wolfeii | |
0.02 | - |
N-formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
0.02 | - |
N-formylmethanofuran | molybdenum enzyme | Methanosarcina barkeri | |
0.03 | - |
N-formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
0.03 | - |
N-formylmethanofuran | - |
Methanothermobacter wolfeii | |
0.035 | - |
formate | molybdenum enzyme | Methanothermobacter wolfeii | |
0.053 | - |
N-Furfurylformamide | molybdenum enzyme | Methanothermobacter wolfeii | |
1.7 | - |
formate | molybdenum enzyme | Methanosarcina barkeri | |
3 | - |
Formamide | molybdenum enzyme | Methanosarcina barkeri | |
5 | - |
methylformamide | molybdenum enzyme | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanosarcina barkeri | |
Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter thermautotrophicus | |
Molybdenum | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii | |
Tungsten | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter thermautotrophicus | |
Tungsten | enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein | Methanothermobacter wolfeii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter wolfeii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Formamide + oxidized acceptor | molybdenum enzyme, 0.1% of the activity relative to formylmethanofuran | Methanosarcina barkeri | ? | - |
? | |
Formate + oxidized acceptor | molybdenum enzyme, 1% of the activity relative to formylmethanofuran | Methanosarcina barkeri | ? | - |
? | |
Formate + oxidized acceptor | molybdenum enzyme, 3% of the activity relative to formylmethanofuran | Methanothermobacter wolfeii | ? | - |
? | |
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | - |
Methanosarcina barkeri | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | tungsten enzymes are specific for N-formylmethanofuran | Methanothermobacter thermautotrophicus | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | tungsten enzymes are specific for N-formylmethanofuran | Methanothermobacter wolfeii | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | molybdenum enzyme is specific for N-formylmethanofuran | Methanothermobacter thermautotrophicus | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
N-Furfurylformamide + oxidized acceptor | - |
Methanothermobacter thermautotrophicus | ? | - |
? | |
N-Furfurylformamide + oxidized acceptor | molybdenum enzyme, 1% of the activity relative to formylmethanofuran | Methanothermobacter wolfeii | ? | - |
? | |
N-Furfurylformamide + oxidized acceptor | molybdenum enzyme, 11% of the activity relative to formylmethanofuran | Methanosarcina barkeri | ? | - |
? | |
N-Methylformamide + oxidized acceptor | molybdenum enzyme, 0.2% of the activity relative to formylmethanofuran | Methanosarcina barkeri | ? | - |
? |