Cloned (Comment) | Organism |
---|---|
- |
Sulfolobus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
pyruvate | pH 6.8, 50°C | Sulfolobus sp. | |
0.87 | - |
2-oxoglutarate | pH 6.8, 50°C | Sulfolobus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains one [4Fe-4S]2+,1+ cluster per alpha/beta structure | Sulfolobus sp. | |
Mg2+ | contains 1.4 mol of Mg per mol of protein | Sulfolobus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus sp. | P72578 and P72579 | P72578: subunit alpha, P72579: subunit beta | - |
Sulfolobus sp. 7 | P72578 and P72579 | P72578: subunit alpha, P72579: subunit beta | - |
Oxidation Stability | Organism |
---|---|
purified enzyme is stable against oxygen | Sulfolobus sp. |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus sp. |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme can be stored after dialysis against 20 mM potassium phosphate buffer, pH 6.8 | Sulfolobus sp. |
20°C, 4°C or -80°C, activity in cytosolic fraction is not very stable | Sulfolobus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + CoA + oxidized ferredoxin | - |
Sulfolobus sp. | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxobutyrate + CoA + oxidized ferredoxin | - |
Sulfolobus sp. 7 | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
Sulfolobus sp. | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
Sulfolobus sp. 7 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | Vmax/Km is 1.9fold higher than that for 2-oxoglutarate | Sulfolobus sp. | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | Vmax/Km is 1.9fold higher than that for 2-oxoglutarate | Sulfolobus sp. 7 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Sulfolobus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Sulfolobus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | the enzyme contains one thiamine diphosphate per alphabeta structure | Sulfolobus sp. |