General Stability | Organism |
---|---|
the operational stability of the enzyme in alginate hydrogels retains over 68% initial activity after 10 repeated cycles | Citrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
CoA | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. | |
0.12 | - |
pyruvate | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. | |
0.37 | - |
pyruvate | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. | |
0.54 | - |
CoA | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM used in assay conditions | Citrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Citrobacter sp. |
240000 | - |
gel filtration | Citrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter sp. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme shows high O2-sensitivity | Citrobacter sp. |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Citrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is incapable of using NAD+ and NADP+ as electron acceptors | Citrobacter sp. | ? | - |
? | |
pyruvate + CoA + oxidized methyl viologen | - |
Citrobacter sp. | acetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 120000, SDS-PAGE | Citrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
PFOR | - |
Citrobacter sp. |
pyruvate ferredoxin oxidoreductase | - |
Citrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Citrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme retains more than 60% of the initial activity 30-50°C, even after 30 min incubation | Citrobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
pyruvate | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. | |
39 | - |
CoA | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. | |
94 | - |
pyruvate | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. | |
109 | - |
CoA | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Citrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme retains more than 70% of the initial activity at pH 6.0-8.0, even after 30 min incubation | Citrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Citrobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
72 | - |
CoA | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. | |
95 | - |
pyruvate | enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C | Citrobacter sp. | |
780 | - |
pyruvate | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. | |
3900 | - |
CoA | free enzyme, at pH 7.0 and 30°C | Citrobacter sp. |