Any feedback?
Literature summary for 1.2.7.1 extracted from
- Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus (1993), Biochim. Biophys. Acta, 1161, 19-27.
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme, stored for one year under liquid N2, no loss of activity if thawed anaerobically | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carbon monoxide | 0.0072 mM, 50% inhibition | Pyrococcus furiosus |  |
| nitrite | - |
Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.094 | - |
oxidized ferredoxin | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.11 | - |
CoA | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.46 | - |
pyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 8.3 gatoms/100000 g of protein | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 31000 | - |
1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 45000 | - |
1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 100000 | - |
electrophoresis on non-denaturing gels | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + 2 oxidized ferredoxin | Pyrococcus furiosus | - |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
pH 8.4, 80°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with 2-oxoglutarate, phenyl pyruvate or indole pyruvate | Pyrococcus furiosus | ? | - |
? | |
| pyruvate + CoA + 2 oxidized ferredoxin | - |
Pyrococcus furiosus | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
| pyruvate + CoA + oxidized methyl viologen | - |
Pyrococcus furiosus | acetyl-CoA + CO2 + reduced methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyruvate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
50% loss of activity after 23 min | Pyrococcus furiosus |
| 90 | - |
50% loss of activity after 18 min | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | 0.5-0.7 per mol of enzyme | Pyrococcus furiosus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
nitrite | pH 8.4, 80°C | Pyrococcus furiosus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
