KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
CoA | pH 7.6, 70°C | Hydrogenobacter thermophilus | |
3.45 | - |
pyruvate | pH 7.6, 70°C | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24500 | - |
1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE | Hydrogenobacter thermophilus |
29000 | - |
1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE | Hydrogenobacter thermophilus |
31500 | - |
1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE | Hydrogenobacter thermophilus |
46000 | - |
1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE | Hydrogenobacter thermophilus |
135000 | - |
gel filtration | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2 oxidized ferredoxin | Hydrogenobacter thermophilus | ferredoxin is an electron donor for the enzyme reaction in vivo | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ferredoxin is an electron donor for the enzyme reaction in vivo | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is inactivated by oxygen, which makes it difficult to purify the enzyme under oxic conditions. The enzyme can be stabilized when 0.1% Triton X-100 is added to the purification buffers | Hydrogenobacter thermophilus |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.46 | - |
pH 7.6, 70°C | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + CoA + oxidized methyl viologen | the enzyme reacts with 2-oxobutyrate and oxaloacetate at 38% activity relative to the activity against pyruvate | Hydrogenobacter thermophilus | propanoyl-CoA + CO2 + reduced methyl viologen | - |
? | |
2-oxobutyrate + CoA + oxidized methyl viologen | the enzyme reacts with 2-oxobutyrate and oxaloacetate at 38% activity relative to the activity against pyruvate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | propanoyl-CoA + CO2 + reduced methyl viologen | - |
? | |
additional information | no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate | Hydrogenobacter thermophilus | ? | - |
? | |
additional information | no activity with 2-oxoglutarate, oxomalonate, 2-oxoisocaproate, phosphoenolpyruvate, indole-3-pyruvate, or phenylpyruvate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? | |
oxaloacetate + CoA + oxidized methyl viologen | the enzyme reacts with 2-oxobutyrate and oxaloacetate at 38% activity relative to the activity against pyruvate | Hydrogenobacter thermophilus | ? | - |
? | |
oxaloacetate + CoA + oxidized methyl viologen | the enzyme reacts with 2-oxobutyrate and oxaloacetate at 38% activity relative to the activity against pyruvate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | ferredoxin is an electron donor for the enzyme reaction in vivo | Hydrogenobacter thermophilus | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | ferredoxin is an electron donor for the enzyme reaction in vivo | Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + oxidized methyl viologen | specific activity with methyl viologen is 10fold higher compared to the specific activity with Hydrogenobacter thermophilus ferredoxin | Hydrogenobacter thermophilus | acetyl-CoA + CO2 + reduced methyl viologen | - |
? | |
pyruvate + CoA + oxidized methyl viologen | specific activity with methyl viologen is 10fold higher compared to the specific activity with Hydrogenobacter thermophilus ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + CO2 + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 46000 + 1 * 31500 + 1 * 29000 + 1 * 24500, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
pyruvate:ferredoxin oxidoreductase | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 85 | - |
Hydrogenobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
8 h, anoxic conditions, 50% loss of activity | Hydrogenobacter thermophilus |
75 | - |
30 min, anoxic conditions, more than 80% residual activity after heating | Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 7.8 | - |
Hydrogenobacter thermophilus |