Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.5.1 extracted from

  • Williams, F.R.; Hager, L.P.
    A crystalline flavin pyruvate oxidase (1961), J. Biol. Chem., 236, PC36-PC37.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystalline enzyme does not contain thiamine diphosphate but has an absolute thiamine diphosphate requirement for the reduction of the enzyme-bound FAD Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
265000
-
determined by the Archibald method Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified enzyme, after crystallization, 6000 Units/mg protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + ferricyanide + H2O
-
Escherichia coli acetate + CO2 + ferrocyanide
-
?
pyruvate + ferricytochrome b1 + H2O
-
Escherichia coli acetate + CO2 + ferrocytochrome b1
-
?

Synonyms

Synonyms Comment Organism
pyruvate oxidase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
ferricyanide without activation of the flavoprotein by proteolytic reactions, i.e. trypsin or chymotrypsin, pH and temperature not specified in the publication Escherichia coli
167
-
pyruvate pH and temperature not specified in the publication Escherichia coli
883
-
ferricyanide with activation of the flavoprotein by proteolytic reactions, i.e. trypsin or chymotrypsin, pH and temperature not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD
-
Escherichia coli