Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D296A | inactive enzyme, no ability of component E1 apoenzyme to reconstitute with thiamine diphosphate | Rattus norvegicus |
H292A | inactive enzyme, no binding of thiamine diphosphate | Rattus norvegicus |
R288A | inactive enzyme, no detectable phosphorylation | Rattus norvegicus |
S293A | 10fold increased Km-value for 3-methyl-2-oxobutanoate, 2.8fold increased Km-value for 4-methyl-2-oxopentanoate, no change in Km-value for thiamine diphosphate | Rattus norvegicus |
S293E | no ability of the E1 apoenzyme to reconstitute with thiamine diphosphate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
3-methyl-2-oxobutanoate | wild-type enzyme and mutant enzyme D297A | Rattus norvegicus | |
0.058 | - |
3-methyl-2-oxobutanoate | mutant enzyme T294A | Rattus norvegicus | |
0.059 | - |
3-methyl-2-oxobutanoate | mutant enzyme G290A and S298A | Rattus norvegicus | |
0.062 | - |
3-methyl-2-oxobutanoate | mutant enzyme I289A and H291A | Rattus norvegicus | |
0.064 | - |
3-methyl-2-oxobutanoate | mutant enzyme S295A | Rattus norvegicus | |
0.568 | - |
3-methyl-2-oxobutanoate | mutant enzyme S293A | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Rattus norvegicus | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Rattus norvegicus | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Rattus norvegicus | |
NAD+ | - |
Rattus norvegicus |