Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
S313A | twofold increase in Km-value but no change in turnover-number | Pseudomonas putida |
S315A | mutation has no effect on Km-value or turnover-number | Pseudomonas putida |
S319A | mutation has no effect on Km-value or turnover-number | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
172000 | - |
equilibrium sedimentation | Pseudomonas putida |
190000 | - |
E1 component, gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Pseudomonas putida |
38 | - |
E1 component expressed in Escherichia coli | Pseudomonas putida |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2,beta2, MW of alpha is 45000 Da, expression of bkdA2 yields the 39000 Da form and the 37000 Da form of the beta-subunit, both forms of the E1 beta-subunit are produced in Pseudomonas putida, only the 39000 Da protein is produced in Escherichia coli, SDS-PAGE | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Pseudomonas putida | |
NAD+ | - |
Pseudomonas putida |