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Literature summary for 1.2.4.4 extracted from

  • Hester, K.L.; Luo, J.; Sokatch, J.R.
    Purification of Pseudomonas putida branched-chain keto acid dehydrogenase E1 component (2000), Methods Enzymol., 324, 129-138.
    View publication on PubMed
Search for more literature for 1.2.4.4

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate
-
 Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
167000
-
 E1 component, equilibrium sedimentation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida enzyme complex is induced by growth on branched-chain amino acid or keto acid and repressed by growth in the presence of glucose or ammonium ion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
E1 component, recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme complex is induced by growth on branched-chain amino acid or keto acid and repressed by growth in the presence of glucose or ammonium ion Pseudomonas putida ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Pseudomonas putida
NAD+
-
 Pseudomonas putida