Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km: 0.00035 mM in the reaction with 4-methyl-2-oxopentanoate | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methyl-2-oxobutanoate | substrate inhibition | Bos taurus | |
3-methylbutanoyl-CoA | competitive with CoA | Bos taurus | |
4-methyl-2-oxopentanoate | substrate inhibition | Bos taurus | |
D-3-methyl-2-oxopentanoate | substrate inhibition | Bos taurus | |
L-3-Methyl-2-oxopentanoate | substrate inhibition | Bos taurus | |
NADH | competitive with NAD+ | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
4-methyl-2-oxopentanoate | - |
Bos taurus | |
0.0156 | - |
3-methyl-2-oxobutanoate | - |
Bos taurus | |
0.0172 | - |
DL-3-methyl-2-oxopentanoate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus | |
Mg2+ | Km: 0.0042 mM in the reaction with 4-methyl-2-oxopentanoate | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Bos taurus | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | D-3-methyl-2-oxopentanoate and L-3-methyl-2-oxopentanoate | Bos taurus | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2 | - |
? | |
4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Bos taurus | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | Km: 0.0071 mM in the reaction with 4-methyl-2-oxopentanoate, Km: 0.009 mM in the reaction with 3-methyl-2-oxobutanoate, Km: 0.007 in the reaction with DL-3-methyl-2-oxopentanoate | Bos taurus | |
NAD+ | Km: 0.109 mM in the reaction with 4-methyl-2-oxopentanoate, Km: 0.126 mM in the reaction with 3-methyl-2-oxobutanoate, Km: 0.101 mM in the reaction with DL-3-methyl-2-oxobutanoate | Bos taurus |