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Literature summary for 1.2.4.1 extracted from

  • Fries, M.; Jung, H.I.; Perham, R.N.
    Reaction mechanism of the heterotetrameric (alpha2beta2) E1 component of 2-oxo acid dehydrogenase multienzyme complexes (2003), Biochemistry, 42, 6996-7002.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
H128A mutation in subunit E1beta, residue His128 provides the proton required to protonate the incoming dithiolane ring in the reductive acetylation of the lipoyl goup Geobacillus stearothermophilus
H271A mutation in subunit E1alpha, residue His271 stabilizes the dianion formed during decarboxylation of the 2-oxo acid Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
pyruvate wild-type, pH 7.0, 30°C Geobacillus stearothermophilus
0.442
-
pyruvate mutant H271A, pH 7.0, 30°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + oxidized 2,6-dichlorophenolindophenol
-
Geobacillus stearothermophilus acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
pyruvate wild-type, pH 7.0, 30°C Geobacillus stearothermophilus
3.8
-
pyruvate mutant H271A, pH 7.0, 30°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate kcat/Km value for wild-type 20 per mM and s, for mutant H271A 950 per mM and s Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
pyruvate mutant H271A, pH 7.0, 30°C Geobacillus stearothermophilus
427
-
pyruvate wild-type, pH 7.0, 30°C Geobacillus stearothermophilus