Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
H128A | mutation in subunit E1beta, residue His128 provides the proton required to protonate the incoming dithiolane ring in the reductive acetylation of the lipoyl goup | Geobacillus stearothermophilus |
H271A | mutation in subunit E1alpha, residue His271 stabilizes the dianion formed during decarboxylation of the 2-oxo acid | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
0.442 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + oxidized 2,6-dichlorophenolindophenol | - |
Geobacillus stearothermophilus | acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
3.8 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | kcat/Km value for wild-type 20 per mM and s, for mutant H271A 950 per mM and s | Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
427 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus |