Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JRG 3456 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 15-20% PEG2000 monomethyl ether, 10% propanol, 0.2% NaN3 in 60 mM HEPES buffer (pH 7.05) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E401A | 9.56% of overall wild type activity and 23.4% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
E401K | 1.04% of overall wild type activity and 4.63% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
H407A | 0.15% of overall wild type activity and 12.0% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K403A | 11.6% of overall wild type activity and 98.0% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K403E | 0.56% of overall wild type activity and 5.46% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K410A | 23.0% of overall wild type activity and 31.6% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K410E | 3.7% of overall wild type activity and 26.1% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K411A | 68.2% of overall wild type activity and 77.5% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
K411E | 38.0% of overall wild type activity and 88.0% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
N404A | 1.81% of overall wild type activity and 45.1% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
Q408A | 31.5% of overall wild type activity and 112.1% of wild type activity with 2,6-dichloroindolphenol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1304 | - |
pyruvate | mutant enzyme Q408A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.1714 | - |
pyruvate | mutant enzyme K410A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.2012 | - |
pyruvate | mutant enzyme K411E, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.2336 | - |
pyruvate | mutant enzyme K411A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.2609 | - |
pyruvate | wild type enzyme, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.6286 | - |
pyruvate | mutant enzyme E401A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.7008 | - |
pyruvate | mutant enzyme K403A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AFG8 | - |
- |
Purification (Comment) | Organism |
---|---|
TSK DEAE-5PW HPLC column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol | - |
Escherichia coli | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E1ec | - |
Escherichia coli |
pyruvate dehydrogenase complex E1 component | - |
Escherichia coli |
pyruvate:NAD oxidoreductase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
pyruvate | mutant enzyme H407A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.21 | - |
pyruvate | mutant enzyme K403E, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
0.39 | - |
pyruvate | mutant enzyme E401K, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
1.53 | - |
pyruvate | mutant enzyme K410E, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
3.07 | - |
pyruvate | mutant enzyme N404A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
3.64 | - |
pyruvate | mutant enzyme E401A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
4.41 | - |
pyruvate | mutant enzyme K403A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
7.68 | - |
pyruvate | mutant enzyme K410A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
19.03 | - |
pyruvate | mutant enzyme Q408A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
25.12 | - |
pyruvate | mutant enzyme K411E, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
35.13 | - |
pyruvate | mutant enzyme K411A, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli | |
37.9 | - |
pyruvate | wild type enzyme, with 2,6-dichlorophenolindophenol, at 30°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Escherichia coli |