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Literature summary for 1.2.4.1 extracted from

  • Hamada, M.; Hiraoka, T.; Koike, K.; Ogasahara, K.; Kanzaki, T.; Koike, M.
    Properties and subunit structure of pig heart pyruvate dehydrogenase (1976), J. Biochem., 79, 1273-1285.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-Dithiopyridine 1.1 mM, 30% inhibition Sus scrofa
5,5'-dithiobis(2-nitrobenzoate) 1.1 mM, 35% inhibition Sus scrofa
AgNO3 18 mol per mol enzyme, complete inhibition Sus scrofa
Aluminium sulfate 1.1 mM, 35% inhibition Sus scrofa
Ba2+ 0.11 mM, 10% inhibition Sus scrofa
Cd2+ 0.032 mM, complete inhibition Sus scrofa
citrate noncompetitive inhibition Sus scrofa
Cu2+ 0.36 mM, complete inhibition Sus scrofa
EDTA 0.036 mM, 40% inhibition, 0.36 mM, complete inhibition, reversed by addition of excess Mg2+ and Ca2+ Sus scrofa
Mn2+ 0.72 mM Sus scrofa
N-ethylmaleimide 1.1 mM, 50% inhibition Sus scrofa
p-chloromercuribenzenesulfonate 8 mol per mol enzyme, complete inhibition Sus scrofa
p-chloromercuribenzoate 8 mol per mol enzyme, complete inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
thiamine diphosphate in the presence of Ca2+ and Mg2+ Sus scrofa
0.14
-
pyruvate in the presence of Ca2+ and Mg2+ Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace Mg2+ Sus scrofa
Ca2+ 0.36 mM, 5fold activation Sus scrofa
Mg2+ 0.36 mM, 5fold activation Sus scrofa
Mn2+ can replace Mg2+ Sus scrofa
Mn2+ 0.11 mM, 2fold activation Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
calculation from sedimentation and diffusion data, low speed sedimentation equilibrium centrifugation, meniscus depletion method Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + lipoamide Sus scrofa in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide Sus scrofa low reactivity with 2-ketovalerate S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide Sus scrofa low reactivity with 2-ketoisovalerate S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide Sus scrofa low reactivity with 2-ketoisocaproate S-acetyldihydrolipoamide + CO2
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pyruvate dehydrogenase Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-18°C, 50 mM potassium phosphate, pH 7.0, 0.5 mM EDTA, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + benzaldehyde
-
Sus scrofa (R)-phenylacetylcarbinol
-
?
alpha-ketobutyrate + lipoamide
-
Sus scrofa S-propionyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate Sus scrofa S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide low reactivity with 2-ketovalerate Sus scrofa S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide low reactivity with 2-ketoisovalerate Sus scrofa S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide low reactivity with 2-ketoisocaproate Sus scrofa S-acetyldihydrolipoamide + CO2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
1 min, complete inactivation Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 with Ca2+ as divalent cation Sus scrofa
6.5 7 with Mg2+ as divalent cation Sus scrofa

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.6
-
citrate
-
Sus scrofa