Literature summary for 1.2.3.4 extracted from

Opaleye, O.; Rose, R.S.; Whittaker, M.M.; Woo, E.J.; Whittaker, J.W.; Pickersgill, R.W.
Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase (2006), J. Biol. Chem., 281, 6428-6433.

Activating Compound

Activating Compound	Comment	Organism	Structure
Formamide	specific activity of the wild type oxalate oxidase is slightly higher when 1 M formamide is added	Hordeum vulgare

Cloned(Commentary)

Cloned (Comment)	Organism
the mutant proteins expressed in Picha pastoris	Hordeum vulgare

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method at 18 °C using 1 micro l of protein (10-15 mg/ml) plus 1 micro l reservoir drops an 1-ml reservoirs, native crystals grown from 2.3M (NH4)2SO4 and 5% 2-propanol are rhombohedral, crystals of recombinant protein, grown from 10% polyethylene glycol 4000 and 0.1 M NaAc, pH 4.6, are tetragonal, Asn75-> Ala OXO are grown from 20% 2-propanol, 0.1 M NaAc, pH 4.6, 0.2 M CaCl2, can be cryo-cooled directly and are rhombohedral	Hordeum vulgare

Crystallization (Comment)

Organism

hanging-drop vapor diffusion method at 18 °C using 1 micro l of protein (10-15 mg/ml) plus 1 micro l reservoir drops an 1-ml reservoirs, native crystals grown from 2.3M (NH4)2SO4 and 5% 2-propanol are rhombohedral, crystals of recombinant protein, grown from 10% polyethylene glycol 4000 and 0.1 M NaAc, pH 4.6, are tetragonal, Asn75-> Ala OXO are grown from 20% 2-propanol, 0.1 M NaAc, pH 4.6, 0.2 M CaCl2, can be cryo-cooled directly and are rhombohedral

Hordeum vulgare

Protein Variants

Protein Variants	Comment	Organism
N75A	reduced activity to 2.4% of the wild type level	Hordeum vulgare
N85A	reduced activity to 0.5% of the wild type level	Hordeum vulgare

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	for wild type oxalate oxidase, glycolate does not serve as a substrate and does not significantly inhibit turnover when included in the assay at equimolar (20mM) concentrations of oxalate	Hordeum vulgare

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NH4Cl	Hordeum vulgare
NH4Cl	specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NaCl	Hordeum vulgare

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	P45850	barley	-

Purification (Commentary)

Purification (Comment)	Organism
mutant proteins	Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.055	-	asparagine mutant (N85A), thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is higher in the presence of 1 M NH4Cl or 1 M NaCl and slightly lower when 1 M formamide is added	Hordeum vulgare
0.24	-	asparagine mutant (N75A),thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is slightly higher in the presence of 1 M NH4Cl or 1 M NaCl and does not change when 1 M formamide is added	Hordeum vulgare
10.26	-	wild type, thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	glycolate does not serve as a substrate	Hordeum vulgare	?	-	?

Synonyms

Synonyms	Comment	Organism
OXO	-	Hordeum vulgare