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Literature summary for 1.2.3.3 extracted from
- A novel biosensor based on activation effect of thiamine on the activity of pyruvate oxidase (2008), Biosens. Bioelectron., 23, 1874-1877.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | pyrodoxin, nicotinic acid, and riboflavin have no activating effects | Aerococcus sp. | |
| additional information | pyrodoxin, riboflavin and nicotinic acid do not play an activator role | Aerococcus sp. | |
| thiamine | 0.0001 mM thiamine leads to 18.2% increase in activity | Aerococcus sp. |  |
| thiamine | activates the enzyme. Can be used as an activator on the biosensor responses | Aerococcus sp. | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | development of a biosensor based on POX enzyme for the investigation of the effect of thiamine (vitamin B1) molecule on the activity of the enzyme | Aerococcus sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Aerococcus sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aerococcus sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cell membrane-associated electron transport system, which includes both ubiquinone-6 and cytochrome b, is the natural electron acceptor for the reduced enzyme and oxygen is the terminal electron acceptor of this system | Aerococcus sp. | ? | - |
? | |
| pyruvate + phosphate + O2 | - |
Aerococcus sp. | acetyl phosphate + CO2 + H2O2 | - |
? | |
| pyruvate + phosphate + O2 + H2O | - |
Aerococcus sp. | acetyl phosphate + CO2 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POX | - |
Aerococcus sp. |
| pyruvate oxidase | - |
Aerococcus sp. |
| pyruvate:oxygen-2-oxidoreductase (phosphorylating) | - |
Aerococcus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Aerococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aerococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aerococcus sp. | |
| thiamine diphosphate | - |
Aerococcus sp. | |
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Release 2023.1 (January 2023)
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