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Literature summary for 1.2.1.94 extracted from

  • Seman-Kamarulzaman, A.; Mohamed-Hussein, Z.; Ng, C.; Hassan, M.
    Novel NAD+-farnesal dehydrogenase from Polygonum minus leaves. Purification and characterization of enzyme in juvenile hormone III biosynthetic pathway in plant (2016), PLoS ONE, 11, e0161707.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.94

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.1 mM, 15% residual activity Persicaria minor
4-chloromercuribenzoate 0.1 mM, 21% residual activity Persicaria minor
Ag+ 0.1 mM, no residual activity Persicaria minor
Ca2+ 0.1 mM, 2% residual activity Persicaria minor
Cu2+ 0.1 mM, no residual activity Persicaria minor
Fe3+ 0.1 mM, 15% residual activity Persicaria minor
iodoacetamide 0.1 mM, 32% residual activity Persicaria minor
Li+ 0.1 mM, 26% residual activity Persicaria minor
Sodium azide 0.1 mM, 20% residual activity Persicaria minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 (2E,6E)-farnesal pH 9.5, 35°C Persicaria minor
0.31
-
 NAD+ pH 9.5, 35°C Persicaria minor
0.69
-
 citral pH 9.5, 35°C Persicaria minor
0.86
-
 trans-cinnamaldehyde pH 9.5, 35°C Persicaria minor
1.28
-
 alpha-methyl-cinnamaldehyde pH 9.5, 35°C Persicaria minor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 1 * 70000, SDS-PAGE Persicaria minor

Organism

Organism UniProt Comment Textmining
Persicaria minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Persicaria minor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Persicaria minor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesal + NAD+ + H2O
-
 Persicaria minor (2E,6E)-farnesoate + NADH + 2 H+
-
 ?
alpha-methyl-cinnamaldehyde + NAD+ + H2O 31% of the activity with farnesal Persicaria minor alpha-methyl-cinnamic acid + NADH + 2 H+
-
 ?
citral + NAD+ + H2O 27% of the activity with farnesal Persicaria minor 3,7-dimethyl-2,6-octadienoate + NADH + 2 H+
-
 ?
trans-cinnamaldehyde + NAD+ + H2O 33% of the activity with farnesal Persicaria minor trans-cinnamic acid + NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Persicaria minor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Persicaria minor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45
-
 rapid inactivation above Persicaria minor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Persicaria minor

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Persicaria minor

pI Value

Organism Comment pI Value Maximum pI Value
Persicaria minor isoelectric focusing
-
 6.6