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Literature summary for 1.2.1.94 extracted from
- Aldehyde dehydrogenase 3 converts farnesal into farnesoic acid in the corpora allata of mosquitoes (2013), Insect Biochem. Mol. Biol., 43, 675-682.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| farnesal | massive accumulation of the potentially toxic farnesal can stimulate the activity of a farnesal reductase or another enzyme that could convert farnesal back into farnesol | Aedes aegypti |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Aedes aegypti |
| five recombinant His-tagged AaALDH3s, representing AaALDH3-2 and the 4 splice variants of AaALDH3-1, PA, PB, PC, PD, overexpressed in Escherichia coli | Aedes aegypti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesal + NAD+ | Aedes aegypti | NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid | (2E,6E)-farnesoate + NADH + 2 H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| (2E,6E)-farnesal + NAD+ | Aedes aegypti Rockefeller | NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid | (2E,6E)-farnesoate + NADH + 2 H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aedes aegypti | - |
- |
- |
| Aedes aegypti | - |
Dengue mosquito | - |
| Aedes aegypti Rockefeller | - |
Dengue mosquito | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corpora allata | - |
Aedes aegypti | - |
| corpus allatum | presents the five transcripts, with their relative proportions changing during development | Aedes aegypti | - |
| hindgut | AaALDH3-1-RC transcript abundance, quantified by qPCR | Aedes aegypti | - |
| malpighian tubule | AaALDH3-1-RB transcript abundance, quantified by qPCR | Aedes aegypti | - |
| additional information | AaALDH3-2 gene has comparatively lower levels of transcript | Aedes aegypti | - |
| nervous system | AaALDH3-1-RD transcript abundance, quantified by qPCR | Aedes aegypti | - |
| ovary | AaALDH3-1-RA transcript abundance, quantified by qPCR | Aedes aegypti | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no activity of AaALDH3-1-PA with decanal, no activity of AaALDH3-1-PA, AaALDH3-1-PD, and AaALDH3-2 with NADP+ | Aedes aegypti |
| 7.08 | - |
recombinant AaALDH3-1-PB with farnesal and NADP+ as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 7.13 | - |
recombinant AaALDH3-1-PC with farnesal and NADP+ as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 103.7 | - |
recombinant AaALDH3-1-PD with decanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 116.8 | - |
recombinant AaALDH3-1-PB with octanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 132.5 | - |
recombinant AaALDH3-2 with decanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 152.7 | - |
recombinant AaALDH3-1-PA with octanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 168.1 | - |
recombinant AaALDH3-1-PD with octanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 203.9 | - |
recombinant AaALDH3-2 with octanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 209.2 | - |
recombinant AaALDH3-2 with farnesal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 214.6 | - |
recombinant AaALDH3-1-PC with octanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 268 | - |
recombinant AaALDH3-1-PD with farnesal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 269.1 | - |
recombinant AaALDH3-1-PB with farnesal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 272.9 | - |
recombinant AaALDH3-1-PA with farnesal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 287.1 | - |
recombinant AaALDH3-1-PB with decanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 362.4 | - |
recombinant AaALDH3-1-PC with decanal as substrate, pH 9.5 and 37°C | Aedes aegypti |
| 468.2 | - |
recombinant AaALDH3-1-PC with farnesal as substrate, pH 9.5 and 37°C | Aedes aegypti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesal + NAD+ | NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid | Aedes aegypti | (2E,6E)-farnesoate + NADH + 2 H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| (2E,6E)-farnesal + NAD+ | NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid | Aedes aegypti Rockefeller | (2E,6E)-farnesoate + NADH + 2 H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| (2E,6E)-farnesal + NAD+ + H2O | - |
Aedes aegypti | (2E,6E)-farnesoic acid + NADH + H+ | - |
? | |
| decanal + NAD+ + H2O | - |
Aedes aegypti | decanoic acid + NADH + H+ | - |
? | |
| decanal + NAD+ + H2O | - |
Aedes aegypti | decanoic acid + NADH + H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| decanal + NAD+ + H2O | - |
Aedes aegypti Rockefeller | decanoic acid + NADH + H+ | - |
? | |
| decanal + NAD+ + H2O | - |
Aedes aegypti Rockefeller | decanoic acid + NADH + H+ | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| additional information | no activity with NADP+ | Aedes aegypti | ? | - |
? | |
| additional information | no activity with NADP+ | Aedes aegypti Rockefeller | ? | - |
? | |
| octanal + NAD+ | - |
Aedes aegypti | octanoic acid + NADH | reaction products analyzed by LC-MS and identified based on the diagnostic ions | ? | |
| octanal + NAD+ + H2O | - |
Aedes aegypti | octanoic acid + NADH + H+ | - |
? | |
| octanal + NAD+ + H2O | - |
Aedes aegypti Rockefeller | octanoic acid + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AaALDH3-1 | - |
Aedes aegypti |
| aldehyde dehydrogenase 3 | - |
Aedes aegypti |
| ALDH3 | - |
Aedes aegypti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Aedes aegypti | |
| NAD+ | could not be substituted by NADP+ | Aedes aegypti | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Aedes aegypti | injection of dsRNAs resulted in a approx. 80% significant reduction of AaALDH3-1 and AaALDH3-2 mRNAs, treatment with dsRNA for the AaALDH3-1 gene results in stronger reductions of farnesoic acid and juvenile hormone than treatment with dsRNA for the AaALDH3-2 gene | down |
| Aedes aegypti | in the presence of an excess of farnesal and NAD+, highly active glands produces 10 times more farnesoic acid than suppressed glands, 24 h after blood feeding | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | results suggest that in the female Dengue mosquito Aedes aegypti, AaALDH3 enzyme plays a critical role in the regulation of juvenile hormone biosynthesis after blood feeding | Aedes aegypti |
| physiological function | the enzyme plays a key role in the regulation of juvenile hormone synthesis in blood-fed mosquito females | Aedes aegypti |
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