Activating Compound | Comment | Organism | Structure |
---|---|---|---|
6-O-phosphono-D-fructofuranose | minor stimulatory effect | Saccharolobus solfataricus | |
AMP | minor stimulatory effect | Saccharolobus solfataricus | |
D-fructose | minor stimulatory effect | Saccharolobus solfataricus | |
D-glucose 1-phosphate | 14fold activation at 0.1 mM D-glucose 1-phosphate | Saccharolobus solfataricus | |
pyruvate | minor stimulatory effect | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
cloned and expressed in BL21 (DE3) CodonPlus using the pET expression vector system | Saccharolobus solfataricus |
expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
expressed in Escherichia coli BL21(DE3) CodonPlus cells | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | slight inhibition | Saccharolobus solfataricus | |
galactonate | slight inhibition; slight inhibition (1.2 to 1.3fold); slight inhibition (1.2 to 1.3fold) | Saccharolobus solfataricus | |
gluconate | slight inhibition | Saccharolobus solfataricus | |
additional information | glyceraldehyde and ADP have no effect on GAPN activity; not inhibited by glyceraldehyde and ADP; not inhibited by glyceraldehyde and ADP | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADP+ | 70°C, pH 7 | Saccharolobus solfataricus | |
0.09 | - |
NADP+ | in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C | Saccharolobus solfataricus | |
0.09 | - |
NADP+ | in the absence or presence of D-glucose 1-phosphate | Saccharolobus solfataricus | |
0.501 | - |
DL-glyceraldehyde 3-phosphate | in the presence of D-glucose 1-phosphate | Saccharolobus solfataricus | |
0.51 | - |
DL-glyceraldehyde 3-phosphate | 70°C, pH 7 | Saccharolobus solfataricus | |
0.51 | - |
D-Glyceraldehyde-3-phosphate | in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C | Saccharolobus solfataricus | |
0.51 | - |
DL-glyceraldehyde 3-phosphate | in the absence of D-glucose 1-phosphate | Saccharolobus solfataricus | |
17.4 | - |
NAD+ | 70°C, pH 7 | Saccharolobus solfataricus | |
17.4 | - |
NAD+ | in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C | Saccharolobus solfataricus | |
17.4 | - |
NAD+ | in the absence of D-glucose 1-phosphate | Saccharolobus solfataricus | |
21.1 | - |
NAD+ | in the presence of D-glucose 1-phosphate | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56930 | - |
subunit, calculated from amino acid sequence | Saccharolobus solfataricus |
57000 | - |
subunit, SDS-PAGE | Saccharolobus solfataricus |
57000 | - |
x * 57000, SDS-PAGE | Saccharolobus solfataricus |
57000 | - |
4 * 57000, SDS-PAGE | Saccharolobus solfataricus |
189000 | - |
- |
Saccharolobus solfataricus |
189000 | - |
native enzyme | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Saccharolobus solfataricus | the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is a key-enzyme of the semi-phosphorylative branch of the EntnerDoudoroff pathway | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | Q97U30 | - |
- |
Saccharolobus solfataricus | Q97XA5 | isozyme Gap-N2 | - |
Saccharolobus solfataricus | Q97XS9 | isozyme GapN-1 | - |
Saccharolobus solfataricus P2 | Q97U30 | - |
- |
Saccharolobus solfataricus P2 | Q97XA5 | isozyme Gap-N2 | - |
Saccharolobus solfataricus P2 | Q97XS9 | isozyme GapN-1 | - |
Purification (Comment) | Organism |
---|---|
heat precipitation and Superdex 200 gel filtration | Saccharolobus solfataricus |
Superdex 200 gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme has a specific activity of 3.74 U/mg | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NAD+ + H2O | enzyme shows negligible activity with NAD+. The NAD+-dependent reaction shows no saturation at NAD+ concentrations up to 50 mM. The highest enzyme activity is observed at 50 mM NAD+ which is 7fold to 8fold lower than the Vmax observed using NADP+ as cofactor | Saccharolobus solfataricus | 3-phospho-D-glycerate + NADH + H+ | - |
ir | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus | 3-phospho-D-glycerate + NADPH + H+ | - |
ir | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is a key-enzyme of the semi-phosphorylative branch of the EntnerDoudoroff pathway | Saccharolobus solfataricus | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
ir | |
D-glyceraldehyde-3-phosphate + NAD+ | weak reaction | Saccharolobus solfataricus | D-3-phosphoglycerate + NADH + H+ | - |
ir | |
D-glyceraldehyde-3-phosphate + NAD+ | weak reaction | Saccharolobus solfataricus P2 | D-3-phosphoglycerate + NADH + H+ | - |
ir | |
D-glyceraldehyde-3-phosphate + NADP+ | - |
Saccharolobus solfataricus | D-3-phosphoglycerate + NADPH + H+ | - |
ir | |
D-glyceraldehyde-3-phosphate + NADP+ | - |
Saccharolobus solfataricus P2 | D-3-phosphoglycerate + NADPH + H+ | - |
ir | |
DL-glyceraldehyde 3-phosphate + NAD+ + H2O | NADP+ is much more efficient than NAD+ | Saccharolobus solfataricus | 3-phospho-DL-glycerate + NADH + H+ | - |
ir | |
DL-glyceraldehyde 3-phosphate + NAD+ + H2O | NADP+ is much more efficient than NAD+ | Saccharolobus solfataricus P2 | 3-phospho-DL-glycerate + NADH + H+ | - |
ir | |
DL-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus | 3-phospho-DL-glycerate + NADPH + H+ | - |
ir | |
DL-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | 3-phospho-DL-glycerate + NADPH + H+ | - |
ir | |
DL-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus | 3-phospho-D-glycerate + NADPH + H+ | - |
r | |
additional information | the enzyme shows only negligible activity with NAD+ | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme shows only negligible activity with NAD+ | Saccharolobus solfataricus P2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Saccharolobus solfataricus |
homotetramer | 4 * 57000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
GAP dehydrogenase | - |
Saccharolobus solfataricus |
GAPN | - |
Saccharolobus solfataricus |
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Saccharolobus solfataricus |
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase GAPN | - |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme shows negligible activity with NAD+. The NAD+-dependent reaction of Sso-GAPN shows no saturation at NAD+ concentrations up to 50 mM. The highest enzyme activity is observed at 50 mM NAD+ which is 7fold to 8fold lower than the Vmax observed using NADP+ as cofactor | Saccharolobus solfataricus | |
NAD+ | negligible activity with NAD+ | Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus | |
NADP+ | prefers NADP+ more than NAD+ as cofactor | Saccharolobus solfataricus | |
NADP+ | the enzyme shows only negligible activity with NAD+ compared to the physiological cosubstrate NADP+ | Saccharolobus solfataricus |