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Literature summary for 1.2.1.9 extracted from
- Expression, purification, and characterization of recombinant nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum (2002), Protein Expr. Purif., 25, 519-526.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL-21 | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 1 mM, 76% inhibition | Clostridium acetobutylicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
NADP+ | pH 8.5, 25°C | Clostridium acetobutylicum | |
| 0.545 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C | Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
4 * 50000, SDS-PAGE | Clostridium acetobutylicum |
| 204000 | - |
non-denaturing PAGE | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
ATCC 824 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | - |
Clostridium acetobutylicum | D-3-phosphoglycerate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Clostridium acetobutylicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Clostridium acetobutylicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, inactivation above | Clostridium acetobutylicum |
| 95 | - |
10 min, complete inactivation | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Clostridium acetobutylicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | absolute specificity | Clostridium acetobutylicum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium acetobutylicum | chromatofocusing | - |
4.2 |
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