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Literature summary for 1.2.1.9 extracted from
- Engineered nonphosphorylating gylceraldehyde 3-phosphate dehydrogenase at position 268 binds hydroxylamine and hydrazine as acyl acceptors (2001), Eur. J. Biochem., 268, 5764-5770.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E268A | attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine | Streptococcus mutans |
| E268Q | attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine | Streptococcus mutans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydrazin | protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism | Streptococcus mutans |  |
| hydroxylamine | protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism | Streptococcus mutans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | Streptococcus mutans | - |
D-3-phosphoglycerate + NADPH | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mutans | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | two-step mechanism and kinetics | Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | - |
Streptococcus mutans | D-3-phosphoglycerate + NADPH | - |
ir | |
| D-glyceraldehyde-3-phosphate + NADP+ | two-step mechanism | Streptococcus mutans | D-3-phosphoglycerate + NADPH | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptococcus mutans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Streptococcus mutans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
steady-state rate constant for mutant E268Q and E268A is pH-dependent | Streptococcus mutans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Streptococcus mutans | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
hydroxylamine | wild-type enzyme | Streptococcus mutans | |
| 6.3 | - |
hydrazin | wild-type enzyme | Streptococcus mutans | |
| 57 | - |
hydroxylamine | mutant E268Q | Streptococcus mutans | |
| 95 | - |
hydroxylamine | mutant E268A | Streptococcus mutans | |
| 109 | - |
hydrazin | mutant E268A | Streptococcus mutans | |
| 254 | - |
hydrazin | mutant E268Q | Streptococcus mutans | |
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Release 2023.1 (January 2023)
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