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Literature summary for 1.2.1.89 extracted from

  • Steffler, F.; Sieber, V.
    Refolding of a thermostable glyceraldehyde dehydrogenase for application in synthetic cascade biomanufacturing (2013), PLoS One, 8, e70592.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.89

Application

Application Comment Organism
synthesis the enzyme accept NAD+ as cofactor under technically relevant conditions, making it suitable for application in the artificial glycolysis by synthetic cascade biomanufacturing Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment) Organism
the gene of TaAlDH is synthesized and cloned into a T7 based vector system for recombinant expression in Escherichia coli. Using matrix-assisted refolding of inclusion bodies the yield of enzyme production is enhanced 43fold Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 NADP+ pH 6.2, 50°C Thermoplasma acidophilum

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C Thermoplasma acidophilum
isobutanol the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HK01
-
-
Thermoplasma acidophilum JCM 9062 Q9HK01
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde + NADP+ + H2O also accepts NAD+ under technically relevant conditions, cofactor above 1 mM Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
D-glyceraldehyde + NADP+ + H2O also accepts NAD+ under technically relevant conditions, cofactor above 1 mM Thermoplasma acidophilum JCM 9062 D-glycerate + NADPH + H+
-
 ?
additional information no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose Thermoplasma acidophilum ?
-
 ?
additional information no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose Thermoplasma acidophilum JCM 9062 ?
-
 ?

Synonyms

Synonyms Comment Organism
Ta0809 locus name Thermoplasma acidophilum
TaAlDH
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 assay at Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NADP+ also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C Thermoplasma acidophilum